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Lead citrate is a compound of lead and citrate that is primarily used as an enhancer for heavy metal staining in electron microscopy. [2] This salt binds to osmium and uranyl acetate and enhances contrast in many cellular structures. Lead citrate is highly reactive with carbon dioxide.
A Ziehl–Neelsen stain is an acid-fast stain used to stain species of Mycobacterium tuberculosis that do not stain with the standard laboratory staining procedures such as Gram staining. This stain is performed through the use of both red coloured carbol fuchsin that stains the bacteria and a counter stain such as methylene blue .
Lead Exposure Elimination Project (LEEP) is an international non-governmental organization aimed at reducing lead exposure in low- and middle-income countries. [1] Based in London, England , it was founded in 2020 to promote research and advocacy on the prevalence and consequences of lead poisoning in the developing world. [ 1 ]
There are various lead abatement techniques to remove residential lead-based paint and lead in household dusts. Encapsulation and enclosure makes the hazard of lead-based paint inaccessible, while chemical stripping, removal of abrasives, scraping with the hand, and component replacement are effective in permanently removing lead-based paints from households. [5]
Kinyoun stain; For color blind people (or in backgrounds where detecting red bacteria is difficult), Victoria blue can be substituted for carbol fuchsin and picric acid can be used as the counter stain instead of methylene blue, and the rest of the Kinyoun technique can be used. [6] Various bacterial spore staining techniques using Kenyon e.g.
Lead poisoning, also known as plumbism and saturnism, is a type of metal poisoning caused by lead in the body. [2] Symptoms may include abdominal pain, constipation, headaches, irritability, memory problems, infertility, numbness and tingling in the hands and feet. [1]
Osmium(VIII) oxide is also used as a stain for lipids in optical microscopy. [26] OsO 4 also stains the human cornea (see safety considerations). A sample of cells fixed/stained with osmium tetroxide (black) embedded in epoxy resin (amber). The cells are black as a result of the effects of osmium tetroxide.
Lead(II) acetate can be made by boiling elemental lead in acetic acid and hydrogen peroxide. This method will also work with lead(II) carbonate or lead(II) oxide. Pb + H 2 O 2 + 2 H + → Pb 2+ + 2 H 2 O Pb 2+ + 2 AcO − → Pb(OAc) 2. Lead(II) acetate can also be made by dissolving lead(II) oxide in acetic acid: [7] PbO + 2 AcOH → Pb(OAc) 2 ...