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The destaining solution for CBB contains usually the buffer salt ammonium bicarbonate (NH 4 HCO 3) and a fraction of 30%-50% organic solvent (mostly acetonitrile). The hydrophobic interactions between protein and CBB are reduced by the organic fraction of the solution. [8]
Although the ammonium bicarbonate protocol should not be considered a non-destructive method, it was followed by more ‘true’ non-destructive methods. The first of these was the eraser protocol, first tested on parchment, [5] but later also applied to bone. [6] The eraser protocol is performed by rubbing a PVC eraser on a piece of parchment ...
Ammonium bicarbonate is an irritant to the skin, eyes and respiratory system. Short-term health effects may occur immediately or shortly after exposure to ammonium bicarbonate. Breathing ammonium bicarbonate can irritate the nose, throat and lungs causing coughing, wheezing and/or shortness of breath.
Activity-based protein profiling (ABPP, also activity-based proteomics) is a technique that was developed to monitor the availability of enzymatic active sites to their endogenous ligands. [5] ABPP uses specially designed probes that enter and form a covalent bond with an enzyme's active site, which confirms that the enzyme is an active state. [7]
BCA protein assay in a 96 well plate. The bicinchoninic acid assay (BCA assay), also known as the Smith assay, after its inventor, Paul K. Smith at the Pierce Chemical Company, [1] now part of Thermo Fisher Scientific, is a biochemical assay for determining the total concentration of protein in a solution (0.5 μg/mL to 1.5 mg/mL), similar to Lowry protein assay, Bradford protein assay or ...
In analytical chemistry, a tandem mass tag (TMT) is a chemical label that facilitates sample multiplexing in mass spectrometry (MS)-based quantification and identification of biological macromolecules such as proteins, peptides and nucleic acids.
CPSI plays a vital role in protein and nitrogen metabolism. Once ammonia has been brought into the mitochondria via glutamine or glutamate, it is CPSI's job to add the ammonia to bicarbonate along with a phosphate group to form carbamoyl phosphate. Carbamoyl phosphate is then put into the urea cycle to eventually create urea.
Most functionally characterized members of the family are ammonium uptake transporters. [11] Some, but not other Amt proteins also transport methylammonium. [12] [13] Detailed phylogenetic analyses of plant homologues have been published. [14] In E. coli, NH 4 +, rather than NH 3, may be the substrate of AmtB, but controversy still exists.
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