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Single-cell sequencing examines the nucleic acid sequence information from individual cells with optimized next-generation sequencing technologies, providing a higher resolution of cellular differences and a better understanding of the function of an individual cell in the context of its microenvironment. [1]
This single cell shows the process of the central dogma of molecular biology, which are all steps researchers are interested to quantify (DNA, RNA, and Protein).. In cell biology, single-cell analysis and subcellular analysis [1] refer to the study of genomics, transcriptomics, proteomics, metabolomics, and cell–cell interactions at the level of an individual cell, as opposed to more ...
Two methods for single-cell ATAC-seq [8]. ATAC-seq stands for Assay for Transposase-Accessible Chromatin with high throughput sequencing. [9] It is a technique used in molecular biology to identify accessible DNA regions, equivalent to DNase I hypersensitive sites. [9]
Within the past five years, the development of single-cell Hi-C has enabled the depiction of the entire 3D structural landscape of chromatins/chromosomes throughout the cell cycle, and many studies have discovered that these identified genomic domains remain unchanged in interphase, and are erased by silencing mechanisms when the cell enters ...
Idiopathic pulmonary fibrosis; Other names: Fibrosing alveolitis, cryptogenic fibrosing alveolitis, diffuse fibrosing alveolitis, usual interstitial pneumonitis, diffuse interstitial pneumonitis: Figure A shows the location of the lungs and airways in the body. The inset image shows a detailed view of the lung's airways and air sacs in cross ...
TCR-Seq (T-cell Receptor Sequencing) is a method used to identify and track specific T cells and their clones. [1] TCR-Seq utilizes the unique nature of a T-cell receptor (TCR) as a ready-made molecular barcode. [1] This technology can apply to both single cell sequencing technologies and high throughput screens [1]
The DNA sequencing is done on a chip that contains many ZMWs. Inside each ZMW, a single active DNA polymerase with a single molecule of single stranded DNA template is immobilized to the bottom through which light can penetrate and create a visualization chamber that allows monitoring of the activity of the DNA polymerase at a single molecule level.
A further consideration when sequencing large, branched cell types, such as neurons, comes from the removal of distal processes containing local pools of RNA during the single-cell isolation process. In these cells, scRNA-seq datasets only capture transcript in the central cell body, omitting transcripts from RNA pools localized to cellular ...