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Very few structures are acid-fast; this makes staining for acid-fastness particularly useful in diagnosis. The following are notable examples of structures which are acid-fast or modified acid-fast: All Mycobacteria – M. tuberculosis, M. leprae, M. smegmatis and atypical mycobacteria.
The acid-fast staining method, in conjunction with auramine phenol staining, serves as the standard diagnostic tool and is widely accessible for rapidly diagnosing tuberculosis (caused by Mycobacterium tuberculosis) and other diseases caused by atypical mycobacteria, such as leprosy (caused by Mycobacterium leprae) and Mycobacterium avium ...
The Kinyoun method can be modified as a weak acid fast stain, which uses 0.5–1.0% sulfuric acid instead of hydrochloric acid.The weak acid fast stain, in addition to staining Mycobacteria, will also stain organisms that are not able to maintain the carbol fuchsin after decolorizing with HCl, such as Nocardia species and Cryptosporidium.
The genus is acid-fast to some degree, it stains only weakly Gram positive. The most common form of human nocardial disease is a slowly progressive pneumonia, the common symptoms of which include cough, dyspnea (shortness of breath), and fever. It is not uncommon for this infection to spread to the pleura or chest wall.
Gram-positive, nonmotile and acid-fast rods (1-3 μm x 0.2-0.4 μm). Sometimes long rods with occasional beaded or swollen cells having non-acid-fast ovoid bodies at one end. Colony characteristics. Smooth hemispheric colonies, usually off-white or cream colored. May be butyrous, waxy, multilobate and even rosette clustered (dilute inocula).
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Mycobacterium leprae is an intracellular, pleomorphic, non-sporing, non-motile, acid-fast, pathogenic bacterium. [3] It is an aerobic bacillus (rod-shaped bacterium) with parallel sides and round ends, surrounded by the characteristic waxy coating of mycolic acid unique to mycobacteria.
Wall sits with calf raises. Stand with your back flat against the wall. Slowly walk your feet away from the wall as you sit down into a squat, bending your knees to a 90-degree angle.Make sure ...