enow.com Web Search

  1. Ad

    related to: agarose gel electrophoresis principle ppt free

Search results

  1. Results from the WOW.Com Content Network
  2. Agarose gel electrophoresis - Wikipedia

    en.wikipedia.org/wiki/Agarose_gel_electrophoresis

    Agarose gel electrophoresis is most commonly done horizontally in a subaquaeous mode whereby the slab gel is completely submerged in buffer during electrophoresis. It is also possible, but less common, to perform the electrophoresis vertically, as well as horizontally with the gel raised on agarose legs using an appropriate apparatus. [ 27 ]

  3. Gel electrophoresis - Wikipedia

    en.wikipedia.org/wiki/Gel_electrophoresis

    Gel electrophoresis of large DNA or RNA is usually done by agarose gel electrophoresis. See the " chain termination method " page for an example of a polyacrylamide DNA sequencing gel. Characterization through ligand interaction of nucleic acids or fragments may be performed by mobility shift affinity electrophoresis .

  4. Gel electrophoresis of proteins - Wikipedia

    en.wikipedia.org/wiki/Gel_electrophoresis_of...

    Proteins separated by SDS-PAGE, Coomassie brilliant blue staining. Protein electrophoresis is a method for analysing the proteins in a fluid or an extract. The electrophoresis may be performed with a small volume of sample in a number of alternative ways with or without a supporting medium, namely agarose or polyacrylamide.

  5. Immunoelectrophoresis - Wikipedia

    en.wikipedia.org/wiki/Immunoelectrophoresis

    Immunoelectrophoresis is a general term describing many combinations of the principles of electrophoresis and reaction of antibodies, also known as immunodiffusion. [1] Agarose as 1% gel slabs of about 1 mm thickness buffered at high pH (around 8.6) is traditionally preferred for electrophoresis and the reaction with antibodies. The agarose was ...

  6. Gel electrophoresis of nucleic acids - Wikipedia

    en.wikipedia.org/wiki/Gel_electrophoresis_of...

    For a standard agarose gel electrophoresis, 0.7% gel concentration gives good separation or resolution of large 5–10kb DNA fragments, while 2% gel concentration gives good resolution for small 0.2–1kb fragments.

  7. Affinity electrophoresis - Wikipedia

    en.wikipedia.org/wiki/Affinity_electrophoresis

    an example of an agarose gel after electrophoresis. A type of electrophoretic mobility shift assay (AMSA), agarose gel electrophoresis is used to separate protein-bound amino acid complexes from free amino acids. Using a low voltage (~10 V/cm) to minimize the risk for heat damage, electricity is run across an agarose gel.

  8. Southern blot - Wikipedia

    en.wikipedia.org/wiki/Southern_blot

    Gel electrophoresis: The DNA fragments are then electrophoresed on an agarose gel to separate them by size. If some of the DNA fragments are larger than 15 kb , then before blotting, the gel may be treated with an acid, such as dilute HCl .

  9. SDD-AGE - Wikipedia

    en.wikipedia.org/wiki/SDD-AGE

    In biochemistry and molecular biology, SDD-AGE is short for Semi-Denaturating Detergent Agarose Gel Electrophoresis. This is a method for detecting and characterizing large protein polymers which are stable in 2% SDS at room temperature, unlike most large protein complexes.

  1. Ad

    related to: agarose gel electrophoresis principle ppt free