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  2. Nicotinamide adenine dinucleotide - Wikipedia

    en.wikipedia.org/wiki/Nicotinamide_adenine_di...

    Both NAD + and NADH strongly absorb ultraviolet light because of the adenine. For example, peak absorption of NAD + is at a wavelength of 259 nanometers (nm), with an extinction coefficient of 16,900 M −1 cm −1. NADH also absorbs at higher wavelengths, with a second peak in UV absorption at 339 nm with an extinction coefficient of 6,220 M ...

  3. P/O ratio - Wikipedia

    en.wikipedia.org/wiki/P/O_ratio

    During normal aerobic respiration the ratio would be somewhere between these values, as the TCA cycle produces both NADH and ubiquinol. The resulting P/O ratio would be the ratio of H/O and H/P; which is 10/3.67 or 2.73 for NADH-linked respiration, and 6/3.67 or 1.64 for UQH2-linked respiration, with actual values being somewhere between.

  4. NADH dehydrogenase - Wikipedia

    en.wikipedia.org/wiki/NADH_dehydrogenase

    NADH dehydrogenase is an enzyme that converts nicotinamide adenine dinucleotide (NAD) from its reduced form (NADH) to its oxidized form (NAD +). Members of the NADH dehydrogenase family and analogues are commonly systematically named using the format NADH:acceptor oxidoreductase .

  5. Respiratory complex I - Wikipedia

    en.wikipedia.org/wiki/Respiratory_complex_I

    NAD + to NADH. FMN to FMNH 2. CoQ to CoQH 2.. Complex I is the first enzyme of the mitochondrial electron transport chain.There are three energy-transducing enzymes in the electron transport chain - NADH:ubiquinone oxidoreductase (complex I), Coenzyme Q – cytochrome c reductase (complex III), and cytochrome c oxidase (complex IV). [1]

  6. Enzyme assay - Wikipedia

    en.wikipedia.org/wiki/Enzyme_assay

    Enzyme activity is a measure of the quantity of active enzyme present and is thus dependent on various physical conditions, which should be specified. It is calculated using the following formula:

  7. Nicotinamide adenine dinucleotide phosphate - Wikipedia

    en.wikipedia.org/wiki/Nicotinamide_adenine_di...

    Nicotinamide adenine dinucleotide phosphate, abbreviated NADP [1] [2] or, in older notation, TPN (triphosphopyridine nucleotide), is a cofactor used in anabolic reactions, such as the Calvin cycle and lipid and nucleic acid syntheses, which require NADPH as a reducing agent ('hydrogen source').

  8. Glycerol-3-phosphate dehydrogenase (NAD+) - Wikipedia

    en.wikipedia.org/wiki/Glycerol-3-phosphate...

    The two substrates of this enzyme are sn-glycerol 3-phosphate and NAD +, whereas its 3 products are glycerone phosphate, NADH, and H +. This enzyme belongs to the family of oxidoreductases , specifically those acting on the CH-OH group of donor with NAD + or NADP + as acceptor.

  9. Monodehydroascorbate reductase (NADH) - Wikipedia

    en.wikipedia.org/wiki/Monodehydroascorbate...

    NADH + H + + 2 monodehydroascorbate NAD + + 2 ascorbate The 3 substrates of this enzyme are NADH , H + , and monodehydroascorbate, whereas its two products are NAD + and ascorbate . This enzyme belongs to the family of oxidoreductases , specifically those acting on NADH or NADPH, with a quinone or similar compound as an acceptor.