Search results
Results from the WOW.Com Content Network
The conditional requirement was the divestment of Thermo Fisher Scientific's cell culture (sera and media), magnetic beads and Dharmacon gene modulation businesses for antitrust reasons. In early January 2014, GE Healthcare reached an agreement with Thermo Fisher Scientific to acquire the cell culture, magnetic bead and Dharmacon gene ...
Minimal essential medium (MEM) is a synthetic cell culture medium developed by Harry Eagle first published in 1959 in Science that can be used to maintain cells in tissue culture. [1] It is based on six salts and glucose described in Earle's salts in 1934: calcium chloride , potassium chloride , magnesium sulfate , sodium chloride , sodium ...
For this purpose, a KlenTaq polymerase was generated by deletion and a phenylalanine at position 667 was exchanged for tyrosine by site-directed mutagenesis (short: F667Y) and named Thermo Sequenase. [47] [48] This polymerase can also be used for the incorporation of fluorescence-labelled dideoxynucleotides. [49]
Invitrogen is one of several brands under the Thermo Fisher Scientific corporation. The product line includes various subbrands of biotechnology products, such as machines and consumables for polymerase chain reaction, reverse transcription, cloning, culturing, stem cell production, cell therapy, regenerative medicine, immunotherapy, transfection, DNA/RNA purification, diagnostic tests ...
A drop of cell culture is placed in the space between the chamber and the glass cover, filling it via capillary action. [1] Looking at the sample under the microscope, the researcher uses the grid to manually count the number of cells in a certain area of known size. The separating distance between the chamber and the cover is predefined, thus ...
HeLa cells are rapidly dividing cancer cells, and the number of chromosomes varies during cancer formation and cell culture. The current estimate (excluding very tiny fragments) is a "hypertriploid chromosome number (3n+)", which means 76 to 80 total chromosomes (rather than the normal diploid number of 46) with 22–25 clonally abnormal ...
Cell culture is a fundamental component of tissue culture and tissue engineering, as it establishes the basics of growing and maintaining cells in vitro. The major application of human cell culture is in stem cell industry, where mesenchymal stem cells can be cultured and cryopreserved for future use. Tissue engineering potentially offers ...
G418 is commonly used in laboratory research to select genetically engineered cells . [2] In general, for bacteria and algae, concentrations of 5 μg/mL or less are used; for mammalian cells, concentrations of approximately 400 μg/mL are used for selection and 200 μg/mL for maintenance.