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  2. Cryogenic electron tomography - Wikipedia

    en.wikipedia.org/wiki/Cryogenic_electron_tomography

    In most other electron microscopy-based methods for imaging biological samples, combining the signal from many different sample copies has been the general way of surpassing this problem (e.g. crystallography, single particle analysis). In cryoET, instead of taking many images of different sample copies, many images are taken of one area.

  3. Cryogenic electron microscopy - Wikipedia

    en.wikipedia.org/wiki/Cryogenic_electron_microscopy

    Cryogenic electron microscopy (cryo-EM) is a cryomicroscopy technique applied on samples cooled to cryogenic temperatures. For biological specimens, the structure is preserved by embedding in an environment of vitreous ice .

  4. Cryomicroscopy - Wikipedia

    en.wikipedia.org/wiki/Cryomicroscopy

    Cryomicroscopy is a technique in which a microscope is equipped in such a fashion that the object intended to be inspected can be cooled to below room temperature. . Technically, cryomicroscopy implies compatibility between a cryostat and a

  5. Transmission electron cryomicroscopy - Wikipedia

    en.wikipedia.org/wiki/Transmission_electron_cryo...

    CryoTEM image of GroEL suspended in amorphous ice at 50 000 × magnification Structure of Alcohol oxidase from Pichia pastoris by CryoTEM. Transmission electron cryomicroscopy (CryoTEM), commonly known as cryo-EM, is a form of cryogenic electron microscopy, more specifically a type of transmission electron microscopy (TEM) where the sample is studied at cryogenic temperatures (generally liquid ...

  6. Scanning electron cryomicroscopy - Wikipedia

    en.wikipedia.org/wiki/Scanning_electron_cryo...

    Scanning electron cryomicroscopy (CryoSEM) is a form of electron microscopy where a hydrated but cryogenically fixed sample is imaged on a scanning electron microscope's cold stage in a cryogenic chamber.

  7. Cryopreservation - Wikipedia

    en.wikipedia.org/wiki/Cryopreservation

    Cryopreservation is a hallmark method for fungi that do not sporulate (otherwise other preservation methods for spores can be used at lower costs and ease), sporulate but have delicate spores (large or freeze-dry sensitive), are pathogenic (dangerous to keep metabolically active fungus) or are to be used for genetic stocks (ideally to have an ...

  8. Transmission electron microscopy - Wikipedia

    en.wikipedia.org/wiki/Transmission_electron...

    Alignment methods use image registration algorithms, such as autocorrelation methods to correct these errors. Secondly, using a reconstruction algorithm, such as filtered back projection , the aligned image slices can be transformed from a set of two-dimensional images, I j ( x , y ), to a single three-dimensional image, I ′ j ( x , y , z ).

  9. Energy filtered transmission electron microscopy - Wikipedia

    en.wikipedia.org/wiki/Energy_filtered...

    Energy-filtered transmission electron microscopy (EFTEM) is a technique used in transmission electron microscopy, in which only electrons of particular kinetic energies are used to form the image or diffraction pattern.