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Chromatographic peak resolution is given by = + where t R is the retention time and w b is the peak width at baseline. The bigger the time-difference and/or the smaller the bandwidths, the better the resolution of the compounds. Here compound 1 elutes before compound 2.
3) Changing α is the most effective way of increasing resolution. This can be done by choosing a stationary phase that has a greater difference between k 1 ' and k 2 '. It can also be done in L.C. by using pH to invoke secondary equilibria (if applicable). The fundamental resolution equation is derived as follows:
FWHM is applied to such phenomena as the duration of pulse waveforms and the spectral width of sources used for optical communications and the resolution of spectrometers. The convention of "width" meaning "half maximum" is also widely used in signal processing to define bandwidth as "width of frequency range where less than half the signal's ...
Two well resolved peaks in a chromatogram. The plate height given as: = with the column length and the number of theoretical plates can be estimated from a chromatogram by analysis of the retention time for each component and its standard deviation as a measure for peak width, provided that the elution curve represents a Gaussian curve.
The Purnell equation is an equation used in analytical chemistry to calculate the resolution R s between two peaks in a chromatogram. [1] [2]= (′ + ′) where R s is the resolution between the two peaks
A major advantage of DLS coupled with SEC is the ability to obtain enhanced DLS resolution. [25] Batch DLS is quick and simple and provides a direct measure of the average size, but the baseline resolution of DLS is a ratio of 3:1 in diameter. Using SEC, the proteins and protein oligomers are separated, allowing oligomeric resolution.
Image resolution is the level of detail of an image. The term applies to digital images, film images, and other types of images. "Higher resolution" means more image detail. Image resolution can be measured in various ways. Resolution quantifies how close lines can be to each other and still be visibly resolved. Resolution units can be tied to ...
The conventional method to evaluate the resolution of a tomography reconstruction is determined by the Crowther criterion. [1] [2] The minimum number of views, m, to reconstruct a particle of diameter D to a resolution of d (=1/R) is given by =.