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This method is much more convenient. However, convenience comes at the cost of specificity and coverage of a wide range of drugs, therefore, HPLC has been used as well as an alternative method. As HPLC is a method of determining (and possibly increasing) purity, using HPLC alone in evaluating concentrations of drugs was somewhat insufficient.
The charged aerosol detector (CAD) is a detector used in conjunction with high-performance liquid chromatography (HPLC) and ultra high-performance liquid chromatography (UHPLC) to measure the amount of chemicals in a sample by creating charged aerosol particles which are detected using an electrometer.
Download as PDF; Printable version; ... High performance liquid chromatography (HPLC) ... List of chemical analysis methods.
Nowadays, LC–MS has become one of the most widely used chemical analysis techniques because more than 85% of natural chemical compounds are polar and thermally labile and GC-MS cannot process these samples. [5] As an example, HPLC–MS is regarded as the leading analytical technique for proteomics and pharmaceutical laboratories.
An evaporative light scattering detector (ELSD) is a destructive chromatography detector, used in conjunction with high-performance liquid chromatography (HPLC), [1] ultra high-performance liquid chromatography (UHPLC), [2] purification liquid chromatography such as flash or preparative chromatography (using a splitter), countercurrent or ...
Summary of analytical methods for chiral analysis Method Brief narrative of principle and application; Chromatographic: Chiral HPLC Chiral HPLC is used to separate enantiomers either by direct or indirect separation mode. Widely employed to check enantiomeric purity, provided the reference standards of the racemate or the two enantiomers are ...
Atmospheric pressure chemical ionization chamber cross section. Atmospheric pressure chemical ionization (APCI) is an ionization method used in mass spectrometry which utilizes gas-phase ion-molecule reactions at atmospheric pressure (10 5 Pa), [1] [2] commonly coupled with high-performance liquid chromatography (HPLC). [3]
Silica gel particles are commonly used as a stationary phase in high-performance liquid chromatography (HPLC) for several reasons, [13] [14] including: High surface area: Silica gel particles have a high surface area, allowing direct interactions with solutes or after bonding of variety of ligands for versatile interactions with the sample molecules, leading to better separations.
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