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  2. SAMtools - Wikipedia

    en.wikipedia.org/wiki/SAMtools

    The index command creates a new index file that allows fast look-up of data in a ... Extract all the reads aligned to the range ... samtools sort -m 5000000 ...

  3. Binary Alignment Map - Wikipedia

    en.wikipedia.org/wiki/Binary_Alignment_Map

    Binary Alignment Map (BAM) is the comprehensive raw data of genome sequencing; [1] it consists of the lossless, compressed binary representation of the Sequence Alignment Map-files. [2] [3] BAM is the compressed binary representation of SAM (Sequence Alignment Map), a compact and index-able representation of nucleotide sequence alignments. [4]

  4. SAM (file format) - Wikipedia

    en.wikipedia.org/wiki/SAM_(file_format)

    The binary equivalent of a SAM file is a Binary Alignment Map (BAM) file, which stores the same data in a compressed binary representation. [4] SAM files can be analysed and edited with the software SAMtools. [1] The header section must be prior to the alignment section if it is present.

  5. List of RNA-Seq bioinformatics tools - Wikipedia

    en.wikipedia.org/wiki/List_of_RNA-Seq...

    The pipeline employs tools like Bowtie, TopHat, ArrayExpressHTS and SAMtools. Also, edgeR or DESeq to perform differential expression. MultiDE; Myrna is a pipeline tool that runs in a cloud environment (Elastic MapReduce) or in a unique computer for estimating differential gene expression in RNA-Seq datasets. Bowtie is employed for short read ...

  6. Pileup format - Wikipedia

    en.wikipedia.org/wiki/Pileup_format

    www.htslib.org /doc /samtools-mpileup.html Pileup format is a text-based format for summarizing the base calls of aligned reads to a reference sequence. This format facilitates visual display of SNP /indel calling and alignment.

  7. Z-order curve - Wikipedia

    en.wikipedia.org/wiki/Z-order_curve

    The Z-ordering can be used to efficiently build a quadtree (2D) or octree (3D) for a set of points. [4] [5] The basic idea is to sort the input set according to Z-order.Once sorted, the points can either be stored in a binary search tree and used directly, which is called a linear quadtree, [6] or they can be used to build a pointer based quadtree.

  8. CRAM (file format) - Wikipedia

    en.wikipedia.org/wiki/CRAM_(file_format)

    Selective access to a CRAM file is granted via the index (with file-name suffix ".crai"). On chromosome and position sorted data this indicates which region is covered by each slice. On unsorted data the index may be used to simply fetch the N th container. Selective decoding may also be achieved using the Compression Header to skip specified ...

  9. Sequence alignment - Wikipedia

    en.wikipedia.org/wiki/Sequence_alignment

    Sequence alignments are also used for non-biological sequences such as calculating the distance cost between strings in a natural language, or to display financial data. A sequence alignment, produced by ClustalO, of mammalian histone proteins. Sequences are the amino acids for residues 120-180 of the proteins. Residues that are conserved ...