Search results
Results from the WOW.Com Content Network
2. Illustration of electrophoresis retardation. Electrophoresis is the motion of charged dispersed particles or dissolved charged molecules relative to a fluid under the influence of a spatially uniform electric field. As a rule, these are zwitterions. [1] Electrophoresis is used in laboratories to separate macromolecules based on their
Gel electrophoresis of nucleic acids is an analytical technique to separate DNA or RNA fragments by size and reactivity. Nucleic acid molecules are placed on a gel , where an electric field induces the nucleic acids (which are negatively charged due to their sugar- phosphate backbone) to migrate toward the positively charged anode .
Electrophoresis, separates organic molecules based on their different interaction with a gel under an electric potential (i.e., different travel) Capillary electrophoresis; Electrostatic separation, works on the principle of corona discharge, where two plates are placed close together and high voltage is applied. This high voltage is used to ...
During electrophoresis in a discontinuous gel system, an ion gradient is formed in the early stage of electrophoresis that causes all of the proteins to focus on a single sharp band in a process called isotachophoresis. Separation of the proteins by size is achieved in the lower, "resolving" region of the gel.
Temperature gradient gel electrophoresis (TGGE) and denaturing gradient gel electrophoresis (DGGE) are forms of electrophoresis which use either a temperature or chemical gradient to denature the sample as it moves across an acrylamide gel. TGGE and DGGE can be applied to nucleic acids such as DNA and RNA, and (less commonly) proteins.
The quantitative principle of affinity electrophoresis illustrated with electrophoresis at pH 8.6 of concanavalin A into an agarose gel containing blood serum (3.6 microliter per square cm). The bar indicates 1 cm. Electrophoresis performed overnight at less than 10 V/cm. The analysis was performed early in the 1970s at the Protein Laboratory
Protein electrophoresis is a method for analysing the proteins in a fluid or an extract. The electrophoresis may be performed with a small volume of sample in a number of alternative ways with or without a supporting medium, namely agarose or polyacrylamide .
Agarose gel electrophoresis is a method of gel electrophoresis used in biochemistry, molecular biology, genetics, and clinical chemistry to separate a mixed population of macromolecules such as DNA or proteins in a matrix of agarose, one of the two main components of agar.