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Electrophoresis is the motion of charged dispersed particles or dissolved charged molecules relative to a fluid under the influence of a spatially uniform electric field. As a rule, these are zwitterions with a positive or negative net charge. [1] Electrophoresis is used in laboratories to separate macromolecules based on their charges.
Polyacrylamide gel electrophoresis (PAGE) is a technique widely used in biochemistry, forensic chemistry, genetics, molecular biology and biotechnology to separate biological macromolecules, usually proteins or nucleic acids, according to their electrophoretic mobility. Electrophoretic mobility is a function of the length, conformation, and ...
Paper chromatography is a useful technique because it is relatively quick and requires only small quantities of material. Separations in paper chromatography involve the principle of partition. In paper chromatography, substances are distributed between a stationary phase and a mobile phase.
Electrophoresis is a method of moving charged particles through a medium by using an electric field induced by electrodes. It is also used to separate molecules with different physical characteristics using electrical charges.
An idealized representation of a solution of a 1:1 electrolyte. A description of Debye–Hückel theory includes a very detailed discussion of the assumptions and their limitations as well as the mathematical development and applications. [5] A snapshot of a 2-dimensional section of an idealized electrolyte solution is shown in the picture.
A molecular-weight size marker, also referred to as a protein ladder, DNA ladder, or RNA ladder, is a set of standards that are used to identify the approximate size of a molecule run on a gel during electrophoresis, using the principle that molecular weight is inversely proportional to migration rate through a gel matrix.
Immunoelectrophoresis is a general term describing many combinations of the principles of electrophoresis and reaction of antibodies, also known as immunodiffusion. [1] Agarose as 1% gel slabs of about 1 mm thickness buffered at high pH (around 8.6) is traditionally preferred for electrophoresis and the reaction with antibodies. The agarose was ...
The moving-boundary electrophoresis apparatus includes a U-shaped cell filled with buffer solution and electrodes immersed at its ends. The sample applied could be any mixture of charged components such as a protein mixture. On applying voltage, the compounds will migrate to the anode or cathode depending on their charges.