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The Nugent Score is a Gram stain scoring system for vaginal swabs to diagnose bacterial vaginosis (BV).The Nugent score is calculated by assessing for the presence of large Gram-positive rods (Lactobacillus morphotypes; decrease in Lactobacillus scored as 0 to 4), small Gram-variable rods (Gardnerella vaginalis morphotypes; scored as 0 to 4), and curved Gram-variable rods (Mobiluncus spp ...
Gram stain (Gram staining or Gram's method), is a method of staining used to classify bacterial species into two large groups: gram-positive bacteria and gram-negative bacteria. It may also be used to diagnose a fungal infection. [1] The name comes from the Danish bacteriologist Hans Christian Gram, who developed the technique in 1884. [2]
Another way to diagnose BV that is less often used in a clinical setting is a Nugent score. To obtain a Nugent score, gram stain techniques are carried out and it characterizes the bacterial morphology on a scale. A score greater than 7 is associated with BV, 4-6 is an intermediate abnormality, and 0-3 is normal. [42]
Violet-stained gram-positive cocci and pink-stained gram-negative bacilli. In bacteriology, gram-positive bacteria are bacteria that give a positive result in the Gram stain test, which is traditionally used to quickly classify bacteria into two broad categories according to their type of cell wall.
Owing to their morphological properties, spirochetes are difficult to Gram-stain but may be visualized using dark field microscopy or Warthin–Starry stain. [35] Examples include: Leptospira species, which cause leptospirosis. Borrelia species, such as Borrelia burgdorferi, a tick-borne bacterium that causes Lyme disease
Curved Gram variable rods – average per high powered (1000× oil immersion) field. View multiple fields (note that this factor is less important – scores of only 0–2 are possible) Score 0 for >30; Score 1 for 15–30; Score 2 for 14; Score 3 for <1 (this is an average, so results can be >0, yet <1) Score 4 for 0; Score 0 for 0
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In pathology, the Grocott–Gömöri's methenamine silver stain, abbreviated GMS, is a popular staining method in histology. The stain was originally named after György Gömöri, the Hungarian physician who developed the stain. It is used widely as a screen for fungal organisms. It is particularly useful in staining carbohydrates.