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Column chromatography in chemistry is a chromatography method used to isolate a single chemical compound from a mixture. Chromatography is able to separate substances based on differential absorption of compounds to the adsorbent; compounds move through the column at different rates, allowing them to be separated into fractions.
In chemistry laboratories, aluminium oxide is a medium for chromatography, available in basic (pH 9.5), acidic (pH 4.5 when in water) and neutral formulations. Additionally, small pieces of aluminium oxide are often used as boiling chips.
Most commercial 99 Mo/ 99m Tc generators use column chromatography, in which 99 Mo in the form of molybdate, MoO 4 2− is adsorbed onto acid alumina (Al 2 O 3). When the 99 Mo decays it forms pertechnetate TcO 4 −, which, because of its single charge, is less tightly bound to the alumina
Reversed-phase liquid chromatography (RP-LC) is a mode of liquid chromatography in which non-polar stationary phase and polar mobile phases are used for the separation of organic compounds. [ 1 ] [ 2 ] [ 3 ] The vast majority of separations and analyses using high-performance liquid chromatography (HPLC) in recent years are done using the ...
This enhances the influence of the column's sulfonic acid functional groups of its surface chemistry over its, slightly diminished (by pH), quaternary amine. Commensurate with this, these analytes will show a reduced retention on the column eluting earlier, and in higher amounts of organic solvent, than if a neutral polar HILIC surface were used.
Filtering the mixture through a plug of silica gel or alumina — if the product is a charged salt, it will remain strongly adsorbed to the silica gel or alumina. [22] Ion exchange chromatography can separate acids, bases, or mixtures of strong and weak acids and bases by their varying affinities to the column medium at different pH. [23]
Most commercial 99 Mo/ 99m Tc generators use column chromatography, in which 99 Mo in the form of water-soluble molybdate, MoO 4 2− is adsorbed onto acid alumina (Al 2 O 3). When the 99 Mo decays, it forms pertechnetate TcO 4 − , which, because of its single charge, is less tightly bound to the alumina.
Activated alumina is manufactured from aluminium hydroxide by dehydroxylating it in a way that produces a highly porous material; this material can have a surface area significantly over 200 m 2 /g. The compound is used as a desiccant (to keep things dry by adsorbing water from the air) and as a filter of fluoride , arsenic and selenium in ...
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