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The different strains have been derived through treating E. coli K-12 with agents such as nitrogen mustard, ultra-violet radiation, X-ray etc. An extensive list of Escherichia coli K-12 strain derivatives and their individual construction, genotypes, phenotypes, plasmids and phage information can be viewed at Ecoliwiki.
E. coli K-12, one of two laboratory strains (innocuous) ... H7 is an enterohemorrhagic strain also 2006 North American E. coli outbreak; E. coli O104:H4, ...
Similarly, other strains of E. coli (e.g. the K-12 strain commonly used in recombinant DNA work) are sufficiently different that they would merit reclassification. A strain is a subgroup within the species that has unique characteristics that distinguish it from other strains. These differences are often detectable only at the molecular level ...
[12] Intestinal mucosa-associated E. coli are observed in increased numbers in the inflammatory bowel diseases, Crohn's disease and ulcerative colitis. [13] Invasive strains of E. coli exist in high numbers in the inflamed tissue, and the number of bacteria in the inflamed regions correlates to the severity of the bowel inflammation. [14]
The LacUV5 promoter is derived from the E. coli wildtype lac promoter but exhibits an increased transcription strength due to two mutations that facilitate its interaction with a native E. coli RNAP σ-factor. [7] In E. coli BL21(DE3) the expression of the T7-RNAP is suppressed by the constitutively expressed LacI repressor.
The E. coli O157:H7 strain that has caused the death of one person is said to cause "very serious disease", especially for the elderly, children and people who are immunocompromised.
RegulonDB is a database of the regulatory network of gene expression in Escherichia coli K-12. [1] [2] RegulonDB also models the organization of the genes in transcription units, operons and regulons. A total of 120 sRNAs with 231 total interactions which all together regulate 192 genes are also included.
Using passive agglutination, Kunin detected the O-antigen found in the lipopolysaccharide (LPS) of E. coli. During the experiments, Kunin noticed that there was cross-reactivity between the rabbit antisera and many of the E. coli strains. One of the sera, O14, reacted to an antigen found in a large range of E. coli strains. Notably, the antigen ...