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Field of view diameter in microscopy. In microscopy, the field of view in high power (usually a 400-fold magnification when referenced in scientific papers) is called a high-power field, and is used as a reference point for various classification schemes.
Apparent or Eye's field of view For telescopes, microscopes, or binoculars, the apparent field of view is a measure of the angular size of the image seen by the eye, through the eyepiece. In other words, it is how large the image appears (as distinct from the magnification).
Scanning electron microscope image of pollen (false colors) Microscopic examination in a biochemical laboratory. Microscopy is the technical field of using microscopes to view objects and areas of objects that cannot be seen with the naked eye (objects that are not within the resolution range of the normal eye). [1]
A high-power field (HPF), when used in relation to microscopy, references the field of view under the maximum magnification power of the objective being used. Often, this represents a 400-fold magnification when referenced in scientific papers.
Bright-field microscopy (BF) is the simplest of all the optical microscopy illumination techniques. Sample illumination is transmitted (i.e., illuminated from below and observed from above) white light , and contrast in the sample is caused by attenuation of the transmitted light in dense areas of the sample.
The eye relief of an optical instrument (such as a telescope, a microscope, or binoculars) is the distance from the last surface of an eyepiece within which the user's eye can obtain the full viewing angle. If a viewer's eye is outside this distance, a reduced field of view will be obtained.
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