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Supercritical fluid chromatography (SFC) [1] is a form of normal phase chromatography that uses a supercritical fluid such as carbon dioxide as the mobile phase. [2] [3] It is used for the analysis and purification of low to moderate molecular weight, thermally labile molecules and can also be used for the separation of chiral compounds.
Supercritical fluid chromatography (SFC) can be used on an analytical scale, where it combines many of the advantages of high performance liquid chromatography (HPLC) and gas chromatography (GC). It can be used with non-volatile and thermally labile analytes (unlike GC) and can be used with the universal flame ionization detector (unlike HPLC ...
It may be a liquid (LC and capillary electrochromatography, CEC), a gas (GC), or a supercritical fluid (supercritical-fluid chromatography, SFC). The mobile phase consists of the sample being separated/analyzed and the solvent that moves the sample through the column.
A chromatography detector is a device that detects and quantifies separated compounds as they elute from the chromatographic column.These detectors are integral to various chromatographic techniques, such as gas chromatography, [1] liquid chromatography, and high-performance liquid chromatography, [2] and supercritical fluid chromatography [3] among others.
Supercritical fluid chromatography (SFC) Principle is very similar to that of HPLC. But SFC typically uses carbon dioxide as the mobile phase. Hence there is a need to pressurize the entire chromatographic flow path. SFC can differentiate enantiomers and enantiomer from the racemate; (+) from (-) and (±) Chiral capillary electrophoresis (CE) [45]
An evaporative light scattering detector (ELSD) is a destructive chromatography detector, used in conjunction with high-performance liquid chromatography (HPLC), [1] ultra high-performance liquid chromatography (UHPLC), [2] purification liquid chromatography such as flash or preparative chromatography (using a splitter), countercurrent or ...
Chromatography software is called also Chromatography Data System. [1] It is located in the data station of the modern liquid, gas or supercritical fluid chromatographic systems. This is a dedicated software connected to an hardware interface within the chromatographic system, which serves as a central hub for collecting, analyzing, and ...
The supercritical solvent is passed into a vessel at lower pressure than the extraction vessel. The density, and hence dissolving power, of supercritical fluids varies sharply with pressure, and hence the solubility in the lower density CO 2 is much lower, and the material precipitates for collection. It is possible to fractionate the dissolved ...