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  2. Phage display - Wikipedia

    en.wikipedia.org/wiki/Phage_display

    4 General protocol. ... Phage display is a laboratory technique for the study of protein–protein, ... "Basics of antibody phage display technology" (PDF).

  3. Phage monographs - Wikipedia

    en.wikipedia.org/wiki/Phage_monographs

    Download as PDF; Printable version; ... Antibody Phage Display: Methods and Protocols. Humana Press, Totawa, NJ. ... Phage Display of Peptides and Proteins: A ...

  4. Biopanning - Wikipedia

    en.wikipedia.org/wiki/Biopanning

    The first step is to have phage display libraries prepared. This involves inserting foreign desired gene segments into a region of the bacteriophage genome, so that the peptide product will be displayed on the surface of the bacteriophage virion. The most often used are genes pIII or pVIII of bacteriophage M13. [5]

  5. Protein engineering - Wikipedia

    en.wikipedia.org/wiki/Protein_engineering

    Phage display methods are one option for screening proteins. This method involves the fusion of genes encoding the variant polypeptides with phage coat protein genes. Protein variants expressed on phage surfaces are selected by binding with immobilized targets in vitro.

  6. Humanized antibody - Wikipedia

    en.wikipedia.org/wiki/Humanized_antibody

    There are technologies that completely avoid the use of mice or other non-human mammals in the process of discovering antibodies for human therapy. Examples of such systems include various "display" methods (primarily phage display) as well as methods that exploit the elevated B-cell levels that occur during a human immune response.

  7. DNA-encoded chemical library - Wikipedia

    en.wikipedia.org/wiki/DNA-encoded_chemical_library

    The design of Halpin and Harbury enabled alternating rounds of selection, PCR amplification and diversification with small organic molecules, in complete analogy to phage display technology. The DNA-routing machinery consists of a series of connected columns bearing resin-bound anticodons, which could sequence-specifically separate a population ...

  8. John McCafferty - Wikipedia

    en.wikipedia.org/wiki/John_McCafferty

    John McCafferty is a British scientist, one of the founders of Cambridge Antibody Technology alongside Sir Gregory Winter and David Chiswell. He is well known as one of the inventors of scFv antibody fragment phage display, [1] a technology that revolutionised the monoclonal antibody drug discovery.

  9. Phage typing - Wikipedia

    en.wikipedia.org/wiki/Phage_typing

    Phage typing is based on the specific binding of phages to antigens and receptors on the surface of bacteria and the resulting bacterial lysis or lack thereof. [4] The binding process is known as adsorption. [5] Once a phage adsorbs to the surface of a bacteria, it may undergo either the lytic cycle or the lysogenic cycle. [6]