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Most organ-on-a-chip models today only culture one cell type, so even though they may be valid models for studying whole organ functions, the systemic effect of a drug on the human body is not verified. In particular, an integrated cell culture analog (μCCA) was developed and included lung cells, drug-metabolizing liver and fat cells.
A recent study based on lab-on-a-chip technology, Digital Dipstick, [28] miniaturised microbiological culture into a dipstick format and enabled it to be used at the point-of-care. Lab-on-a-chip technology can also be useful for the diagnosis and management of viral infections.
Lab disk for protein structure analysis via small-angle X-ray scattering. The centrifugal micro-fluidic biochip or centrifugal micro-fluidic biodisk is a type of lab-on-a-chip technology, also known as lab-on-a-disc, that can be used to integrate processes such as separating, mixing, reaction and detecting molecules of nano-size in a single piece of platform, including a compact disk or DVD.
Perfusion in microfluidic cell culture is important to enable long culture periods on-chip and cell differentiation. [ 16 ] Other critical aspects for controlling the microenvironment include: cell seeding density, reduction of air bubbles as they can rupture cell membranes, evaporation of media due to an insufficiently humid environment, and ...
By including gas exchange and temperature control systems on chip, microfluidic cell culturing can eliminate the need for incubators and tissue culture hoods. [3] However, this type of continuous microfluidic cell culture operation presents its own unique challenges as well.
Confining cells and exerting controlled forces by coupling with external force-generation methods such as Stokes flow, optical tweezer, or controlled deformation of the PDMS (Polydimethylsiloxane) device [82] [83] [84] Electric field integration [84] Plant on a chip and plant tissue culture [85]
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