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  2. DNA extraction - Wikipedia

    en.wikipedia.org/wiki/DNA_extraction

    The first isolation of deoxyribonucleic acid (DNA) was done in 1869 by Friedrich Miescher. [1] DNA extraction is the process of isolating DNA from the cells of an organism isolated from a sample, typically a biological sample such as blood, saliva, or tissue. It involves breaking open the cells, removing proteins and other contaminants, and ...

  3. Cell isolation - Wikipedia

    en.wikipedia.org/wiki/Cell_isolation

    Cell isolation is the process of separating individual living cells from a solid block of tissue or cell suspension. While some types of cell naturally exist in a separated form (for example blood cells ), other cell types that are found in solid tissue require specific techniques to separate them into individual cells.

  4. Primary cell culture - Wikipedia

    en.wikipedia.org/wiki/Primary_cell_culture

    Primary cell culture is the ex vivo culture of cells freshly obtained from a multicellular organism, as opposed to the culture of immortalized cell lines.In general, primary cell cultures are considered more representative of in vivo tissues than cell lines, and this is recognized legally in some countries such as the UK (Human Tissue Act 2004). [1]

  5. Acid guanidinium thiocyanate-phenol-chloroform extraction

    en.wikipedia.org/wiki/Acid_guanidinium...

    This method may take longer than a column-based system such as the silica-based purification, but has higher purity and the advantage of high recovery of RNA. [1] Furthermore, an RNA column is typically unsuitable for purification of short (<200 nucleotides ) RNA species, such as siRNA , miRNA and tRNA .

  6. Laser capture microdissection - Wikipedia

    en.wikipedia.org/wiki/Laser_capture_microdissection

    Under a microscope using a software interface, a tissue section (typically 5-50 micrometres thick) is viewed and individual cells or clusters of cells are identified either manually or in semi-automated or more fully automated ways allowing the imaging and then automatic selection of targets for isolation. Currently six primary isolation ...

  7. Single-cell analysis - Wikipedia

    en.wikipedia.org/wiki/Single-cell_analysis

    This single cell shows the process of the central dogma of molecular biology, which are all steps researchers are interested to quantify (DNA, RNA, and Protein).. In cell biology, single-cell analysis and subcellular analysis [1] refer to the study of genomics, transcriptomics, proteomics, metabolomics, and cellcell interactions at the level of an individual cell, as opposed to more ...

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  9. Bioprocess - Wikipedia

    en.wikipedia.org/wiki/Bioprocess

    The upstream part of a bioprocess refers to the first step in which microbes/cells are grown, e.g. bacterial or mammalian cell lines (see cell culture), in bioreactors. Upstream processing involves all the steps related to inoculum development, media development, improvement of inoculum by genetic engineering process, optimization of growth ...

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