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The yeast deletion project, formally the Saccharomyces Genome Deletion Project, is a project to create data for a near-complete collection of gene-deletion mutants of the yeast Saccharomyces cerevisiae. Each strain carries a precise deletion of one of the genes in the genome. This allows researchers to determine what each gene does by comparing ...
Synthetic genetic array analysis is generally conducted using colony arrays on petriplates at standard densities (96, 384, 768, 1536). To perform a SGA analysis in S.cerevisiae, the query gene deletion is crossed systematically with a deletion mutant array (DMA) containing every viable knockout ORF of the yeast genome (currently 4786 strains). [9]
Gene knockout by mutation is commonly carried out in bacteria. An early instance of the use of this technique in Escherichia coli was published in 1989 by Hamilton, et al. [2] In this experiment, two sequential recombinations were used to delete the gene.
However, marker expression can have polar effects on the expression of upstream and downstream genes. Removal of selectable markers from the genome by Cre-lox recombination is an elegant and efficient way to circumvent this problem and is therefore widely used in plants, mouse cell lines, yeast, etc. [1]
In genetics, Flp-FRT recombination is a site-directed recombination technology, increasingly used to manipulate an organism's DNA under controlled conditions in vivo.It is analogous to Cre-lox recombination but involves the recombination of sequences between short flippase recognition target (FRT) sites by the recombinase flippase (Flp) derived from the 2 μ plasmid of baker's yeast ...
Sequencing of the entire yeast genome has made it possible to generate a library of knock-out mutants for nearly every gene in the genome. These molecularly bar-coded mutants greatly facilitate high-throughput epistasis studies, as they can be pooled and used to generate the necessary double mutants. Both SGA and dSLAM approaches rely on these ...
As Sec14p is an essential gene, Sec14p knockouts must be performed in yeast strains with several other mutations conveying viability without functional Sec14p. In this knockout mutant, certain proteins destined for export accumulate in non-vesicular compartments of the cell.
For example, deletion of a gene by gene targeting (gene knockout) can be done in some organisms, such as yeast, mice and moss. Unique among plants, in Physcomitrella patens , gene knockout via homologous recombination to create knockout moss (see figure) is nearly as efficient as in yeast. [ 4 ]
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