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Phenolphthalein (/ f ɛ ˈ n ɒ l (f) θ ə l iː n / [citation needed] feh-NOL(F)-thə-leen) is a chemical compound with the formula C 20 H 14 O 4 and is often written as "HIn", "HPh", "phph" or simply "Ph" in shorthand notation. Phenolphthalein is often used as an indicator in acid–base titrations.
Phenol red, 40 μM: colors in cell culture medium at a pH range from 6.0 to 8.0. Most living tissues prosper at a near-neutral pH—that is, a pH close to 7. The pH of blood ranges from 7.35 to 7.45, for instance. When cells are grown in tissue culture, the medium in which they grow is held close to this physiological pH. A small amount of ...
Hydrogen ion(pH 7.4) 4 ... Reference ranges for common blood tests; References This page was last edited on 20 March 2023, at 15:48 (UTC). Text is ...
The Kastle–Meyer test is a presumptive blood test, first described in 1903, in which the chemical indicator phenolphthalein is used to detect the possible presence of hemoglobin. It relies on the peroxidase -like activity of hemoglobin in blood to catalyze the oxidation of phenolphthalin (the colorless reduced form of phenolphthalein) into ...
Acid–base and blood gases are among the few blood constituents that exhibit substantial difference between arterial and venous values. [6] Still, pH, bicarbonate and base excess show a high level of inter-method reliability between arterial and venous tests, so arterial and venous values are roughly equivalent for these. [44]
The pH after the equivalence point depends on the concentration of the conjugate base of the weak acid and the strong base of the titrant. However, the base of the titrant is stronger than the conjugate base of the acid. Therefore, the pH in this region is controlled by the strong base. As such the pH can be found using the following: [1]
As with all presumptive tests, this technique can produce false positive results due to metals and strong chemicals, such as bleach, that will also react. Another common presumptive test is the Kastle-Meyer or Phenolphthalein test. This is a catalytic test that detects the heme group in blood that transports oxygen and carbon dioxide. [5]
If pH is below the pK a or pK b value, the converse is true. Usually, the color change is not instantaneous at the pK a or pK b value, but a pH range exists where a mixture of colors is present. This pH range varies between indicators, but as a rule of thumb, it falls between the pK a or pK b value plus or minus one. This assumes that solutions ...
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