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  2. Köhler illumination - Wikipedia

    en.wikipedia.org/wiki/Köhler_illumination

    Köhler illumination is a method of specimen illumination used for transmitted and reflected light (trans- and epi-illuminated) optical microscopy.Köhler illumination acts to generate an even illumination of the sample and ensures that an image of the illumination source (for example a halogen lamp filament) is not visible in the resulting image.

  3. Microscopy with UV surface excitation - Wikipedia

    en.wikipedia.org/wiki/Microscopy_with_UV_surface...

    The microscope setup is based on an inverted microscope design. [ 2 ] [ 3 ] [ 4 ] An automated stage is used to record larger areas by mosaicing a series of single adjacent frames. The LED light is focused using a ball lens with a short focal length onto the sample surface in an oblique-angle cis-illumination scheme since standard microscopy ...

  4. Microscopy - Wikipedia

    en.wikipedia.org/wiki/Microscopy

    A two-photon microscope is also a laser-scanning microscope, but instead of UV, blue or green laser light, a pulsed infrared laser is used for excitation. Only in the tiny focus of the laser is the intensity high enough to generate fluorescence by two-photon excitation , which means that no out-of-focus fluorescence is generated, and no pinhole ...

  5. Condenser (optics) - Wikipedia

    en.wikipedia.org/wiki/Condenser_(optics)

    A condenser is an optical lens that renders a divergent light beam from a point light source into a parallel or converging beam to illuminate an object to be imaged. Condensers are an essential part of any imaging device, such as microscopes, enlargers, slide projectors, and telescopes.

  6. Dark-field microscopy - Wikipedia

    en.wikipedia.org/wiki/Dark-field_microscopy

    Diagram illustrating the light path through a dark-field microscope. The steps are illustrated in the figure where an inverted microscope is used. Light enters the microscope for illumination of the sample. A specially sized disc, the patch stop (see figure), blocks some light from the light source, leaving an outer ring of illumination. A wide ...

  7. Fluorescence microscope - Wikipedia

    en.wikipedia.org/wiki/Fluorescence_microscope

    The wave nature of light limits the size of the spot to which light can be focused due to the diffraction limit. This limitation was described in the 19th century by Ernst Abbe and "limits an optical microscope's resolution to approximately half of the wavelength of the light used." Fluorescence microscopy is central to many techniques which ...

  8. Fluorescence imaging - Wikipedia

    en.wikipedia.org/wiki/Fluorescence_imaging

    Excitation source: a device that produces either a broad-wavelength source like UV light, or a narrow wavelength source like a laser. Light display optics: the mechanism of which light illuminates the sample. This is typically done through direct illumination of the sample. Light assortment optics: the collection method of the light itself.

  9. Bright-field microscopy - Wikipedia

    en.wikipedia.org/wiki/Bright-field_microscopy

    The light path of a bright-field microscope is extremely simple; no additional components are required beyond the normal light-microscope setup. The light path begins at the illuminator or the light source on the base of the microscope. Often a halogen lamp is used. The light travels through the objective lens into the ocular lens, through ...

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