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Video by Tami (Guy) Port, Chief Executive Nerd at ScienceProfOnline.com. For the lab materials that go with this movie, see the Virtual Microbiology Classroom Microscopy Laboratory Main Page at ScienceProfOnline.com. Date: 26 September 2012: Source: Cheek Epithelial Cells: How to Prepare a Wet Mount Microscope Slide: Author: ScienceProfOnline
A microscope slide is a thin flat piece of glass, typically 75 by 26 mm (3 by 1 inches) and about 1 mm thick, used to hold objects for examination under a microscope. Typically the object is mounted (secured) on the slide, and then both are inserted together in the microscope for viewing.
Typically, nitrocellulose slides have a thin, opaque film of nitrocellulose on a standard 25mm × 75 mm glass microscope slide. The film is extremely sensitive to contact, and to foreign material; contact causes deformation and deposition of material, especially liquids. [citation needed]
Once the specimen and water are combined on the slide, the cover glass is added. The cover glass should be placed at an angle to the slide, one edge touching the slide, and then lowered as if hinged there. If done properly, the water will force out any air as the cover glass closes over it, and no bubbles will be trapped beneath the glass ...
The slide is left to air dry, after which the blood is fixed to the slide by immersing it briefly in methanol. The fixative is essential for good staining and presentation of cellular detail. After fixation, the slide is stained to distinguish the cells from each other. [citation needed]
A cytocentrifuge, sometimes referred to as a cytospin, [1] is a specialized centrifuge used to concentrate cells in fluid specimens onto a microscope slide so that they can be stained and examined. [2] Cytocentrifuges are used in various areas of the clinical laboratory, such as cytopathology, hematology and microbiology, as well as in ...
From there the tissue can be mounted on a microscope slide, stained with appropriate aqueous dye(s) after removal of the paraffin, and examined using a light microscope. [ 12 ] Frozen section procedure : water-rich tissues are hardened by freezing and cut in the frozen state with a freezing microtome or microtome- cryostat ; sections are ...
The slide is left to stand until clear, normally between five and fifteen minutes, in order to dissolve skin cells, hair, and debris. To enhance clearing dimethyl sulfoxide can be added to the slide. To make the fungi easier to see lactophenol cotton blue stain can be added. The slide is gently heated to speed up the action of the KOH.