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FISH is widely used in the field of microbial ecology, to identify microorganisms. Biofilms, for example, are composed of complex (often) multi-species bacterial organizations. Preparing DNA probes for one species and performing FISH with this probe allows one to visualize the distribution of this specific species within the biofilm.
Cirrhitidae hawkfishes are mostly too small to be of interest to fisheries. The three largest species are occasionally fished for as food fish. A few of the smaller more colourful species, particularly Neocirrhites armatus and Oxycirrhites typus, are collected for the aquarium trade. [6]
Probe design for CISH is very similar to that for FISH with differences only in labelling and detection. FISH probes are generally labelled with a variety of different fluorescent tags and can only be detected under a fluorescence microscope, [4] whereas CISH probes are labelled with biotin or digoxigenin [5] and can be detected using a bright-field microscope after other treatment steps have ...
The flame hawkfish (Neocirrhites armatus) is a species of marine ray-finned fish, a hawkfish belonging to the family Cirrhitidae. It is native to tropical reefs of the Pacific Ocean at depths of from 1 to 10 metres (3.3 to 32.8 ft). This species is also found in the aquarium trade. It is the only known member of its genus.
Fluorescence in situ hybridization (FISH)is the most widely used riboprobe technique. A target sequence and a probe are essential in FISH. First, the probe is labeled with either direct or indirect labeling strategy: hapten-modified nucleotides are used in indirect labeling, and fluorophore-modified nucleotides are used in direct labeling.
The longnose hawkfish (Oxycirrhites typus) is a species of marine ray-finned fish, a hawkfish belonging to the family Cirrhitidae.It is found on tropical reefs of the Indian Ocean and the Pacific Ocean, where it can be found at depths around 10 to 100 m (33 to 328 ft).
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