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DNA polymerase II (also known as DNA Pol II or Pol II) is a prokaryotic DNA-dependent DNA polymerase encoded by the PolB gene. [1] DNA Polymerase II is an 89.9-kDa protein and is a member of the B family of DNA polymerases. It was originally isolated by Thomas Kornberg in 1970, and characterized over the next few years.
MCM2-7 is required for both DNA replication initiation and elongation; its regulation at each stage is a central feature of eukaryotic DNA replication. [3] During G1 phase, the two head-to-head Mcm2-7 rings serve as the scaffold for the assembly of the bidirectional replication initiation complexes at the replication origin.
This METAR example is from Trenton-Mercer Airport near Trenton, New Jersey, and was taken on 5 December 2003 at 18:53 UTC. METAR KTTN 051853Z 04011KT 1/2SM VCTS SN FZFG BKN003 OVC010 M02/M02 A3006 RMK AO2 TSB40 SLP176 P0002 T10171017= [14] METAR indicates that the following is a standard hourly observation.
In aviation, atmospheric sciences and broadcasting, a height above ground level (AGL [1] or HAGL) is a height measured with respect to the underlying ground surface.This is as opposed to height above mean sea level (AMSL or HAMSL), height above ellipsoid (HAE, as reported by a GPS receiver), or height above average terrain (AAT or HAAT, in broadcast engineering).
The amplification reaction initiates when multiple primer hexamers anneal to the template. When DNA synthesis proceeds to the next starting site, the polymerase displaces the newly produced DNA strand and continues its strand elongation. The strand displacement generates a newly synthesized single-stranded DNA template for more primers to anneal.
DNA polymerase's ability to slide along the DNA template allows increased processivity. There is a dramatic increase in processivity at the replication fork. This increase is facilitated by the DNA polymerase's association with proteins known as the sliding DNA clamp. The clamps are multiple protein subunits associated in the shape of a ring.
The polymerase chain reaction is the most widely used method for in vitro DNA amplification for purposes of molecular biology and biomedical research. [1] This process involves the separation of the double-stranded DNA in high heat into single strands (the denaturation step, typically achieved at 95–97 °C), annealing of the primers to the single stranded DNA (the annealing step) and copying ...
If an extra copy is present in the test sample, the signals are expected to be 1.5 times the intensities of the respective probes from the reference. If only one copy is present the proportion is expected to be 0.5. If the sample has two copies, the relative probe strengths are expected to be equal.