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Pathophysiology sample values; BMP/ELECTROLYTES: Na + = 140 Cl − = 100 BUN = 20 / Glu = 150 \ K + = 4 CO 2 = 22 PCr = 1.0 ARTERIAL BLOOD GAS: HCO 3 − = 24 p a CO 2 = 40 p a O 2 = 95 pH = 7.40 ALVEOLAR GAS: p A CO 2 = 36 p A O 2 = 105 A-a g = 10 OTHER: Ca = 9.5 Mg 2+ = 2.0 PO 4 = 1 CK = 55 BE = −0.36 AG = 16 SERUM OSMOLARITY/RENAL: PMO ...
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Template-switching polymerase chain reaction (TS-PCR) is a method of reverse transcription and polymerase chain reaction (PCR) amplification that relies on a natural PCR primer sequence at the polyadenylation site, also known as the poly(A) tail, and adds a second primer through the activity of murine leukemia virus reverse transcriptase. [1]
RT-PCR. Reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique combining reverse transcription of RNA into DNA (in this context called complementary DNA or cDNA) and amplification of specific DNA targets using polymerase chain reaction (PCR). [1] It is primarily used to measure the amount of a specific RNA.
Inverse polymerase chain reaction (Inverse PCR) is a variant of the polymerase chain reaction that is used to amplify DNA with only one known sequence. One limitation of conventional PCR is that it requires primers complementary to both termini of the target DNA, but this method allows PCR to be carried out even if only one sequence is ...
Real time PCR uses fluorophores in order to detect levels of gene expression. Cells in all organisms regulate gene expression by turnover of gene transcripts (single stranded RNA): The amount of an expressed gene in a cell can be measured by the number of copies of an RNA transcript of that gene present in a sample. In order to robustly detect ...
A strip of eight PCR tubes, each containing a 100 μL reaction mixture Placing a strip of eight PCR tubes into a thermal cycler. The polymerase chain reaction (PCR) is a method widely used to make millions to billions of copies of a specific DNA sample rapidly, allowing scientists to amplify a very small sample of DNA (or a part of it) sufficiently to enable detailed study.
This technique was developed in 1983 by Kary Mullis. PCR is now a common and important technique used in medical and biological research labs for a variety of applications. [19] PCR, or Polymerase Chain Reaction, is a widely used molecular biology technique to amplify a specific DNA sequence. Steps of polymerase chain reaction