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Hematoxylin staining shown as "basophilic" at top, seen with dual staining with hematoxylin and eosin (H&E). Haematoxylin stain is commonly followed (or counterstained) with another histologic stain, eosin. [10] [11] [1] When paired, this staining procedure is known as H&E staining, and is one of the most commonly used combinations in histology.
Hematoxylin and eosin stain (or haematoxylin and eosin stain or hematoxylin-eosin stain; often abbreviated as H&E stain or HE stain) is one of the principal tissue stains used in histology. [ 1 ] [ 2 ] [ 3 ] It is the most widely used stain in medical diagnosis [ 1 ] and is often the gold standard . [ 4 ]
Mayer's reagent is an alkaloidal precipitating reagent used for the detection of alkaloids in natural products. Mayer's reagent is freshly prepared by dissolving a mixture of mercuric chloride (1.36 g) and of potassium iodide (5.00 g) in water (100.0 ml).
Very dilute solutions of aluminium-haematein, used at pH 3.2 (higher than is usual for staining), contain a cationic dye-metal complex and will slowly stain nucleic acids. [2] Haemalum solutions used for routine staining are more concentrated and more acidic (pH 2-2.5) and are able to stain nuclei after chemical or enzymatic extraction of DNA ...
There is much more phosphotungstic acid in the solution than hematein. The phosphotungstic acid binds all of the available hematein to form a blue lake pigment. This lake stains the muscle cross striations, fibrin, nuclei, and other tissue elements blue. The rest of the phosphotungstic acid stains the red-brown components, such as collagen. [2]
The trichrome is applied by immersion of the fixated sample into Weigert's iron hematoxylin, and then three different solutions, labeled A, B, and C: Weigert's hematoxylin is a sequence of three solutions: ferric chloride in diluted hydrochloric acid, hematoxylin in 95% ethanol, and potassium ferricyanide solution alkalized by sodium borate.
The first staining solution contains haematoxylin which stains cell nuclei. [10] [2] [12] Papanicolaou used Harris's hematoxylin in all three formulations of the stain he published. [2] The second staining solution (designated OG-6), contains Orange G in 95% ethyl alcohol with a small amount of phosphotungstic acid.
Hematoxylin and Van Gieson's stain gives collagen a pink color, such as in fibrosis (arrows, here in cirrhosis). Van Gieson's stain is a mixture of picric acid and acid fuchsin . It is the simplest method of differential staining of collagen and other connective tissue .