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The nucleolus ultrastructure can be seen through an electron microscope, while the organization and dynamics can be studied through fluorescent protein tagging and fluorescent recovery after photobleaching . Antibodies against the PAF49 protein can also be used as a marker for the nucleolus in immunofluorescence experiments. [17]
When observed under the electron microscope, the nucleolus can be seen to consist of three distinguishable regions: the innermost fibrillar centers (FCs), surrounded by the dense fibrillar component (DFC) (that contains fibrillarin and nucleolin), which in turn is bordered by the granular component (GC) (that contains the protein nucleophosmin ...
P80-coilin is a specific marker for coiled bodies, [9] and demonstrates these bodies tend to be associated with the nucleolus when cells are not dividing. CBs are associated with telomerase assembly and recruitment via a CAB-RNA sequence common in both CB RNAs ( scaRNAs ) and the RNA component of telomerase (TERC).
The protoplasmic material of the nucleus including the nucleolus labelled as nucleoplasm. The nucleoplasm, also known as karyoplasm, [1] is the type of protoplasm that makes up the cell nucleus, the most prominent organelle of the eukaryotic cell. It is enclosed by the nuclear envelope, also known as the nuclear membrane. [2]
The actual shape of the nuclear envelope is irregular. It has invaginations and protrusions and can be observed with an electron microscope. A volumetric surface render (red) of the nuclear envelope of one HeLa cell. The cell was observed in 300 slices of electron microscopy, the nuclear envelope was automatically segmented and rendered. One ...
The electron microscope can achieve a resolution of up to 100 picometers, allowing eukaryotic cells, prokaryotic cells, viruses, ribosomes, and even single atoms to be visualized (note the logarithmic scale). Transmission electron microscopy DNA sequencing is a single-molecule sequencing technology that uses transmission electron microscopy ...
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