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  2. Radial immunodiffusion - Wikipedia

    en.wikipedia.org/wiki/Radial_immunodiffusion

    [1] [2] The diameter of the circle increases with time as the antigen diffuses into the medium, reacts with the antibody, and forms insoluble precipitin complexes. [ 1 ] [ 2 ] [ 5 ] The antigen is quantitated by measuring the diameter of the precipitin circle and comparing it with the diameters of precipitin circles formed by known quantities ...

  3. RING1 - Wikipedia

    en.wikipedia.org/wiki/RING1

    19763 Ensembl ENSG00000204227 ENSMUSG00000024325 UniProt Q06587 O35730 RefSeq (mRNA) NM_002931 NM_009066 RefSeq (protein) NP_002922 NP_033092 Location (UCSC) Chr 6: 33.21 – 33.21 Mb Chr 17: 34.24 – 34.24 Mb PubMed search Wikidata View/Edit Human View/Edit Mouse E3 ubiquitin-protein ligase RING1 is an enzyme that in humans is encoded by the RING1 gene. Function This gene belongs to the RING ...

  4. Antibody - Wikipedia

    en.wikipedia.org/wiki/Antibody

    Each antibody binds to a specific antigen in a highly specific interaction analogous to a lock and key.. An antibody (Ab) or immunoglobulin (Ig) is a large, Y-shaped protein belonging to the immunoglobulin superfamily which is used by the immune system to identify and neutralize antigens such as bacteria and viruses, including those that cause disease.

  5. Anti-SSA/Ro autoantibodies - Wikipedia

    en.wikipedia.org/wiki/Anti-SSA/Ro_autoantibodies

    Patients with SLE and SS not only show elevated levels of Anti-Ro antibodies, but also elevated levels of Ro52. [3] [10] Ro52 has one primary epitope to which anti-Ro/SSA binds, independent of the autoimmune disease. The most common domain anti-Ro52 targets is the coiled coil (cc) domain, as well as the RING and B-box domains. [5]

  6. Immunoglobulin light chain - Wikipedia

    en.wikipedia.org/wiki/Immunoglobulin_light_chain

    Once set, light chain class remains fixed for the life of the B lymphocyte. In a healthy individual, the total kappa-to-lambda ratio is roughly 2:1 in serum (measuring intact whole antibodies) or 1:1.5 if measuring free light chains, with a highly divergent ratio indicative of neoplasm. The free light chain ratio ranges from 0.26 to 1.65. [1]

  7. Hook effect - Wikipedia

    en.wikipedia.org/wiki/Hook_effect

    The hook effect refers to the prozone phenomenon, also known as antibody excess, or the postzone phenomenon, also known as antigen excess. It is an immunologic phenomenon whereby the effectiveness of antibodies to form immune complexes can be impaired when concentrations of an antibody or an antigen are very high.

  8. Complementarity-determining region - Wikipedia

    en.wikipedia.org/wiki/Complementarity...

    A single antibody molecule has two antigen receptors and therefore contains twelve CDRs total. There are three CDR loops per variable domain in antibodies. Sixty CDRs can be found on a pentameric IgM molecule, which is composed of five antibodies and has increased avidity as a result of the collective affinity of all antigen-binding sites combined.

  9. Primary and secondary antibodies - Wikipedia

    en.wikipedia.org/wiki/Primary_and_secondary...

    This reduces the cost by labeling only one type of secondary antibody, rather than labeling various types of primary antibodies. Secondary antibodies help increase sensitivity and signal amplification due to multiple secondary antibodies binding to a primary antibody. [2]