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[2] [3] Carbol fuchsin is used as the primary stain dye to detect acid-fast bacteria because it is more soluble in the cells' wall lipids than in the acid alcohol. If the bacteria is acid-fast the bacteria will retain the initial red color of the dye because they are able to resist the destaining by acid alcohol (0.4–1% HCl in 70% EtOH). [ 4 ]
Mouse skin stained with Masson's trichrome stain. Masson's trichrome is a three-colour staining procedure used in histology . The recipes emerged from Claude L. Pierre Masson 's (1880–1959) original formulation have different specific applications, but all are suited for distinguishing cells from surrounding connective tissue .
A Ziehl–Neelsen stain is an acid-fast stain used to stain species of Mycobacterium tuberculosis that do not stain with the standard laboratory staining procedures such as Gram staining. This stain is performed through the use of both red coloured carbol fuchsin that stains the bacteria and a counter stain such as methylene blue.
Fungal yeast forms are inconsistently stained with Acid-fast stain which is considered a narrow spectrum stain for fungi. [21] In a study on acid-fastness of fungi, [ 22 ] 60% of blastomyces and 47% of histoplasma showed positive cytoplasmic staining of the yeast-like cells, and Cryptococcus or candida did not stain, and very rare staining was ...
Maneval's capsule stain: the capsule appears as a clear halo between the pink-stained bacterium and the bluish-grey stained background. The background stain is the acidic stain Congo red (which changes color to bluish-grey due to the pH), and the pink stain is fuchsine .
Immunohistochemistry can be performed on tissue that has been fixed and embedded in paraffin, but also cryopreservated (frozen) tissue.Based on the way the tissue is preserved, there are different steps to prepare the tissue for immunohistochemistry, but the general method includes proper fixation, antigen retrieval incubation with primary antibody, then incubation with secondary antibody.
Gastric signet ring cell carcinoma histopathology, PAS stain Esophageal candidiasis, PAS stain Liver in glycogen storage disease, PAS stain. PAS staining is mainly used for staining structures containing a high proportion of carbohydrate macromolecules (glycogen, glycoprotein, proteoglycans), typically found in e.g. connective tissues, mucus, the glycocalyx, and basal laminae.
Bacterial cell envelopes fall into two major categories: a Gram-positive type which stains purple during Gram staining and a Gram-negative type which stains pink during Gram staining. Either type may have an enclosing capsule of polysaccharides for extra protection. As a group these are known as polysaccharide encapsulated bacteria