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  2. Relay lens - Wikipedia

    en.wikipedia.org/wiki/Relay_lens

    In optics, a relay lens is a lens or a group of lenses that receives the image from the objective lens and relays it to the eyepiece. Relay lenses are found in refracting telescopes , endoscopes , and periscopes to optically manipulate the light path , extend the length of the whole optical system , and usually serve the purpose of inverting ...

  3. Köhler illumination - Wikipedia

    en.wikipedia.org/wiki/Köhler_illumination

    Köhler illumination is a method of specimen illumination used for transmitted and reflected light (trans- and epi-illuminated) optical microscopy.Köhler illumination acts to generate an even illumination of the sample and ensures that an image of the illumination source (for example a halogen lamp filament) is not visible in the resulting image.

  4. Polarized light microscopy - Wikipedia

    en.wikipedia.org/wiki/Polarized_light_microscopy

    Polarizing microscope operating principle Depiction of internal organs of a midge larva via birefringence and polarized light microscopy. Polarized light microscopy can mean any of a number of optical microscopy techniques involving polarized light. Simple techniques include illumination of the sample with polarized light.

  5. Optical transfer function - Wikipedia

    en.wikipedia.org/wiki/Optical_transfer_function

    As an example, the figure on the right shows the 3D point-spread function in object space of a wide-field microscope (a) alongside that of a confocal microscope (c). Although the same microscope objective with a numerical aperture of 1.49 is used, it is clear that the confocal point spread function is more compact both in the lateral dimensions ...

  6. Microscopy - Wikipedia

    en.wikipedia.org/wiki/Microscopy

    Antonie van Leeuwenhoek (1632–1723). The field of microscopy (optical microscopy) dates back to at least the 17th-century.Earlier microscopes, single lens magnifying glasses with limited magnification, date at least as far back as the wide spread use of lenses in eyeglasses in the 13th century [2] but more advanced compound microscopes first appeared in Europe around 1620 [3] [4] The ...

  7. Point spread function - Wikipedia

    en.wikipedia.org/wiki/Point_spread_function

    By virtue of the linearity property of optical non-coherent imaging systems, i.e., . Image(Object 1 + Object 2) = Image(Object 1) + Image(Object 2). the image of an object in a microscope or telescope as a non-coherent imaging system can be computed by expressing the object-plane field as a weighted sum of 2D impulse functions, and then expressing the image plane field as a weighted sum of the ...

  8. Dispersion staining - Wikipedia

    en.wikipedia.org/wiki/Dispersion_staining

    The colors seen with this type of dispersion staining are about the same as those with the Becke` Line method shown in Chart 1. Examples of this type of dispersion staining and the colors shown for different λo's can be seen at microlabgallery.com site for Becke` Line Dispersion Staining. The presence of two colors helps to bracket the ...

  9. Stereo microscope - Wikipedia

    en.wikipedia.org/wiki/Stereo_microscope

    The stereo, stereoscopic or dissecting microscope is an optical microscope variant designed for low magnification observation of a sample, typically using light reflected from the surface of an object rather than transmitted through it. The instrument uses two separate optical paths with two objectives and eyepieces to provide slightly ...

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