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In the field of biotechnology, cloning is the process of creating cloned organisms of cells and of DNA fragments. The artificial cloning of organisms, sometimes known as reproductive cloning, is often accomplished via somatic-cell nuclear transfer (SCNT), a cloning method in which a viable embryo is created from a somatic cell and an egg cell.
This technique is currently the basis for cloning animals (such as the famous Dolly the sheep), [30] and has been proposed as a possible way to clone humans. Using SCNT in reproductive cloning has proven difficult with limited success. High fetal and neonatal death make the process very inefficient.
In animals, parthenogenesis means development of an embryo from an unfertilized egg cell. In plants, parthenogenesis is a component process of apomixis. In algae, parthenogenesis can mean the development of an embryo from either an individual sperm or an individual egg.
Cloning animals requires procedures that can cause pain and distress, and there can be high failure and mortality rates.” Being able to produce genetically identical monkeys could be useful ...
Females can produce full clones of themselves through a modification of the normal meiosis process used to produce haploid egg cells for sexual reproduction. The female's germ cells undergo a process of premeiotic genome doubling, or endoreduplication, so that two consecutive division cycles in the process of meiosis result in a diploid, rather than haploid, genome.
Nuclear transfer is a delicate process that is a major hurdle in the development of cloning technology. [5] Materials used in this procedure are a microscope, a holding pipette (small vacuum) to keep the oocyte in place, and a micropipette (hair-thin needle) capable of extracting the nucleus of a cell using a vacuum.
The reprogramming process that cells need to go through during cloning is not perfect and embryos produced by nuclear transfer often show abnormal development. [ 33 ] [ 34 ] Making cloned mammals was highly inefficient – in 1996, Dolly was the only lamb that survived to adulthood from 277 attempts.
Asexual reproduction is not limited to single-celled organisms. The cloning of an organism is a form of asexual reproduction. By asexual reproduction, an organism creates a genetically similar or identical copy of itself. The evolution of sexual reproduction is a major puzzle for biologists. The two-fold cost of sexual reproduction is that only ...
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