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Guanidinium thiocyanate can be used to deactivate a virus, such as the influenza virus that caused the 1918 "Spanish flu", so that it can be studied safely.. Guanidinium thiocyanate is also used to lyse cells and virus particles in RNA and DNA extractions, where its function, in addition to its lysing action, is to prevent activity of RNase enzymes and DNase enzymes by denaturing them.
TRIzol reagent contains guanidinium thiocyanate and phenol.. TRIzol is a widely used [1] chemical solution used in the extraction of DNA, RNA, and proteins from cells. The solution was initially used and published by Piotr Chomczyński and Nicoletta Sacchi in 1987.
Guanidinium thiocyanate is also used for its denaturing effect on various biological samples. Recent studies suggest that guanidinium is produced by bacteria as a toxic byproduct. To alleviate the toxicity of guanidinium, bacteria have developed a class of transporters known as guanidinium exporters or Gdx proteins to expel the extra amounts of ...
Guanidinium chloride is a weak acid with a pK a of 13.6. The reason that it is such a weak acid is the complete delocalization of the positive charge through three nitrogen atoms (plus a little bit of positive charge on carbon). However, some stronger bases can deprotonate it, such as sodium hydroxide: C(NH 2) + 3 + OH − ⇌ HNC(NH 2) 2 + H 2 O
Examples thereof are aqueous solution of: thiocyanate ion, iodine ion, perchlorate ion, nitrate ion, bromine ion, chlorine ion, acetate ion, fluorine ion, and sulfate ion, or mutual combinations therewith. According to the original Boom method, the chaotropic guanidinium salt employed is preferably guanidinium thiocyanate (GuSCN).
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Pineapples are pretty cool plants. Beyond being delicious (and the key ingredient for an amazing tropical cocktail), these spiky fruits also have a lot to offer from a health perspective—and ...
In methyl thiocyanate, N≡C and C−S distances are 116 and 176 pm. By contrast, N=C and C=S distances are 117 and 158 pm in isothiocyanates. [7] Typical bond angles for C−S−C are 100°. [3] By contrast C−N=C in aryl isothiocyanates is 165°. Again, the thiocyanate isomers are quite different with C−S−C angle near 100°.
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