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  2. Binary Alignment Map - Wikipedia

    en.wikipedia.org/wiki/Binary_Alignment_Map

    Binary Alignment Map (BAM) is the comprehensive raw data of genome sequencing; [1] it consists of the lossless, compressed binary representation of the Sequence Alignment Map-files. [ 2 ] [ 3 ] BAM is the compressed binary representation of SAM (Sequence Alignment Map), a compact and index-able representation of nucleotide sequence alignments ...

  3. SAMtools - Wikipedia

    en.wikipedia.org/wiki/SAMtools

    samtools view -bS sample.sam > sample.bam. Convert a sam file into a bam file. The -b option compresses or leaves compressed input data. samtools view sample_sorted.bam "chr1:10-13" Extract all the reads aligned to the range specified, which are those that are aligned to the reference element named chr1 and cover its 10th, 11th, 12th or 13th ...

  4. SAM (file format) - Wikipedia

    en.wikipedia.org/wiki/SAM_(file_format)

    The SAM format consists of a header and an alignment section. [1] The binary equivalent of a SAM file is a Binary Alignment Map (BAM) file, which stores the same data in a compressed binary representation. [4] SAM files can be analysed and edited with the software SAMtools. [1] The header section must be prior to the alignment section if it is ...

  5. Sequence analysis - Wikipedia

    en.wikipedia.org/wiki/Sequence_analysis

    The output from this step, that is, the aligned reads, are stored in compatible file formats known as SAM, which contains information about the reference genome as well as individual reads. Alternatively, BAM file formats are preferred as they use much less desk or storage space. [14]

  6. CRAM (file format) - Wikipedia

    en.wikipedia.org/wiki/CRAM_(file_format)

    Compressed Reference-oriented Alignment Map (CRAM) is a compressed columnar file format for storing biological sequences aligned to a reference sequence, initially devised by Markus Hsi-Yang Fritz et al. [1] CRAM was designed to be an efficient reference-based alternative to the Sequence Alignment Map (SAM) and Binary Alignment Map (BAM) file ...

  7. PacBio (PACB) Enhances Sequencing Platform Via Latest Buyout

    www.aol.com/news/pacbio-pacb-enhances-sequencing...

    PacBio's (PACB) latest acquisition is expected to deliver the most advanced blend of sequencing solutions to transform the genomics landscape.

  8. List of open-source bioinformatics software - Wikipedia

    en.wikipedia.org/wiki/List_of_open-source...

    Centralized web application that provides data format transformations and facilitates connections with other bioinformatics tools Web browser: LGPL: Broad Institute, collaborative project GENtle: An equivalent to the proprietary Vector NTI, a tool to analyze and edit DNA sequence files Linux, macOS, Windows: GPL: Magnus Manske: gget

  9. FASTQ format - Wikipedia

    en.wikipedia.org/wiki/FASTQ_format

    Raw PacBio subreads use the same convention but typically assign a placeholder base quality (Q0) to all bases in the read. [7] Oxford Nanopore Duplex reads, called using the dorado basecaller are typically stored in SAM/BAM format. After changing to a 16-bit internal quality representation, the reported base quality limit is q50 (S). [8]

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