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The blue–white screen is a screening technique that allows for the rapid and convenient detection of recombinant bacteria in vector-based molecular cloning experiments. This method of screening is usually performed using a suitable bacterial strain , but other organisms such as yeast may also be used.
Download QR code; In other projects ... A schematic for the Blue-white assay for E coli. Used to screen for recombinant vectors. ... some details may not fully ...
This allows for blue–white screening when using host strains such as E. coli JM109, which produces only the C-terminal portion of lacZ, also known as the β-polypeptide. [3] If pUC19 is inserted into E. coli JM109 and grown on agar media supplemented with IPTG and X-gal , then colonies will appear blue, as the plasmid encodes for the α ...
DH5-Alpha Cells are E. coli cells engineered by American biologist Douglas Hanahan to maximize transformation efficiency. They are defined by three [1] mutations: recA1, endA1 which help plasmid insertion and lacZΔM15 which enables blue white screening.
A selectable marker is a gene introduced into cells, especially bacteria or cells in culture, which confers one or more traits suitable for artificial selection.They are a type of reporter gene used in laboratory microbiology, molecular biology, and genetic engineering to indicate the success of a transfection or transformation or other procedure meant to introduce foreign DNA into a cell.
If value of 1, the summary data of seats is shown (colored box & number); if value of 2 a list with colored box, party name, and number is shown; dafault value is 0. Background Defines the background color using a hex color. n1, n2, ... Number of seats of each group or party c1, c2, ...
File:Blue-white test.jpg. Add languages. Page contents not supported in other languages. File; Talk; English. ... Download QR code ...
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