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The blue–white screen is a screening technique that allows for the rapid and convenient detection of recombinant bacteria in vector-based molecular cloning experiments. This method of screening is usually performed using a suitable bacterial strain , but other organisms such as yeast may also be used.
This allows for blue–white screening when using host strains such as E. coli JM109, which produces only the C-terminal portion of lacZ, also known as the β-polypeptide. [3] If pUC19 is inserted into E. coli JM109 and grown on agar media supplemented with IPTG and X-gal , then colonies will appear blue, as the plasmid encodes for the α ...
[[Category:Chart, diagram and graph templates]] to the <includeonly> section at the bottom of that page. Otherwise, add <noinclude>[[Category:Chart, diagram and graph templates]]</noinclude> to the end of the template code, making sure it starts on the same line as the code's last character.
width: width of the chart; height: height of the chart; type: type of the chart: line for line charts, area for area charts, and rect for (column) bar charts, and pie for pie charts. Multiple series can stacked using the stacked prefix, e.g. stackedarea. interpolate: interpolation method for line and area charts.
A graph or chart or diagram is a diagrammatical illustration of a set of data. If the graph is uploaded as an image file, it can be placed within articles just like any other image. Graphs must be accurate and convey information efficiently. They should be viewable at different computer screen resolutions.
A selectable marker is a gene introduced into cells, especially bacteria or cells in culture, which confers one or more traits suitable for artificial selection.They are a type of reporter gene used in laboratory microbiology, molecular biology, and genetic engineering to indicate the success of a transfection or transformation or other procedure meant to introduce foreign DNA into a cell.
The N 2 chart or N 2 diagram (pronounced "en-two" or "en-squared") is a chart or diagram in the shape of a matrix, representing functional or physical interfaces between system elements. It is used to systematically identify, define, tabulate, design, and analyze functional and physical interfaces.
A summary is specified ("This is an example chart") and the whole chart's background color is set to blue. The example also shows how the chart's overall style can be overridden by more specific styles set by {}. In this case, the color of the first row of cells is set to yellow using the features of the {} template; see that template's ...
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