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Nucleotide bonds showing AT and GC pairs. Arrows point to the hydrogen bonds.. In molecular biology and genetics, GC-content (or guanine-cytosine content) is the percentage of nitrogenous bases in a DNA or RNA molecule that are either guanine (G) or cytosine (C). [1]
Many sources of bias were already reported – GC content and PCR enrichment, [18] [19] rRNA depletion, [20] errors produced during sequencing, [21] priming of reverse transcription caused by random hexamers. [22] Different tools were developed to attempt to solve each of the detected errors.
TurboMass is GC/MS software created by PerkinElmer. Universal Mass Calculator Freeware: UMC is programmed as a mass spectrometry tool to assist interpreting measurement results, mainly derived from molecular or quasi-molecular ions. It can be used for the calculation of: Mass deviation (mmu or ppm) of measured mass from given empirical formula
OLIGO Primer Analysis Software is a software for DNA primer design. [1] [2] The first paper describing this software was published in 1989. [3] The program is a real time PCR primer and probe search and analysis tool. It additionally performs siRNA and molecular beacon searches, open reading frame analysis, and restriction enzyme analysis.
Chargaff's second rule appears to be the consequence of a more complex parity rule: within a single strand of DNA any oligonucleotide (k-mer or n-gram; length ≤ 10) is present in equal numbers to its reverse complementary nucleotide. Because of the computational requirements this has not been verified in all genomes for all oligonucleotides.
Indication of the origin and terminus of DNA replication on a GC skew and cumulative GC skew plot. Richness of G over T in the leading strand, resulting in a GC skew sign at the origin and terminus. GC skew is when the nucleotides guanine and cytosine are over- or under-abundant in a particular region of DNA or RNA. GC skew is also a ...
Oligonucleotides are short DNA or RNA molecules, oligomers, that have a wide range of applications in genetic testing, research, and forensics.Commonly made in the laboratory by solid-phase chemical synthesis, [1] these small fragments of nucleic acids can be manufactured as single-stranded molecules with any user-specified sequence, and so are vital for artificial gene synthesis, polymerase ...
If calculator gadget is not enabled, should just show the x_default and y_default values. Supports all the same parameters as {{ Superimpose }} except x and y are now formulas, and there are two new parameters: x_default and y_default for the initial x and y values.