Ads
related to: next generation sequencing database
Search results
Results from the WOW.Com Content Network
This design is very different from that of Sanger sequencing—also known as capillary sequencing or first-generation sequencing—which is based on electrophoretic separation of chain-termination products produced in individual sequencing reactions. [6] This methodology allows sequencing to be completed on a larger scale. [7]
SNV calling from NGS data is any of a range of methods for identifying the existence of single nucleotide variants (SNVs) from the results of next generation sequencing (NGS) experiments. These are computational techniques, and are in contrast to special experimental methods based on known population-wide single nucleotide polymorphisms (see ...
In the case of next-generation sequencing methods, library preparation is required before processing. [151] Assessing the quality and quantity of nucleic acids both after extraction and after library preparation identifies degraded, fragmented, and low-purity samples and yields high-quality sequencing data. [152]
Accurately performs gapped alignment of sequence data obtained from next-generation sequencing machines (specifically of Solexa-Illumina) back to a genome of any size. Includes adaptor trimming, SNP calling and Bisulfite sequence analysis. Yes, also supports Illumina *_int.txt and *_prb.txt files with all 4 quality scores for each base
RNA-Seq was first developed in mid 2000s with the advent of next-generation sequencing technology. [144] The first manuscripts that used RNA-Seq even without using the term includes those of prostate cancer cell lines [ 145 ] (dated 2006), Medicago truncatula [ 146 ] (2006), maize [ 147 ] (2007), and Arabidopsis thaliana [ 148 ] (2007), while ...
The main focus of the implementation is on usability and to incorporate read trimming in next-generation sequencing data processing and analysis pipelines. It can process single-end and paired-end sequencing data of arbitrary length. cutadapt [25] removes adapter sequences from next-generation sequencing data (Illumina, SOLiD and 454). It is ...
Ads
related to: next generation sequencing database