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These bacteria cause Mycobacterium avium-intracellulare infections or Mycobacterium avium complex infections in humans. [2] These bacteria are common and are found in fresh and salt water, in household dust and in soil. [3] MAC bacteria usually cause infection in those who are immunocompromised or those with severe lung disease.
[1] [2] Once stained as part of a sample, these organisms can resist the acid and/or ethanol-based decolorization procedures common in many staining protocols, hence the name acid-fast. [ 2 ] The mechanisms of acid-fastness vary by species although the most well-known example is in the genus Mycobacterium , which includes the species ...
Mycobacterium phlei is a species of acid-fast bacteria in the genus Mycobacterium. [1] It is characterized as one of the fast-growing mycobacteria. M. phlei has only occasionally been isolated in human infections, and patients infected with M. phlei generally respond well to anti-mycobacterial therapy. M. phlei has an unusually high GC-content ...
Mycobacterium tuberculosis group bacteria are 1.0-4.0 μm long by 0.2-0.3 μm wide in tissues. In culture, they may appear as cocci, or as bacilli up to 6-8 μm long. [citation needed] The bacteria stain Gram-positive, acid-fast. The cell wall contains as high as 60% lipid, giving the mycobacteria their hydrophobic characteristics, slow growth ...
Initial suspicion of Pott’s Disease is usually based on clinical symptoms and imaging findings, but a definitive diagnosis requires isolating the organism by culture, identifying it, and determining its drug susceptibility. The typical lab procedure for clinical specimens involves an AFB (acid-fast bacilli) stain.
Other bacteria are commonly identified with a microscope by staining them with Gram stain. However, the mycolic acid in the cell wall of M. tuberculosis does not absorb the stain. Instead, acid-fast stains such as Ziehl–Neelsen stain, or fluorescent stains such as auramine are used. [4]
After the Ziehl-Neelsen staining procedure using carbol fuchsin, acid-fast bacteria are observable as vivid red or pink rods set against a blue or green background, depending on the specific counterstain used, such as methylene blue or malachite green, respectively. Non-acid-fast bacteria and other cellular structures will be colored by the ...
The genus is acid-fast to some degree, it stains only weakly Gram positive. The most common form of human nocardial disease is a slowly progressive pneumonia, the common symptoms of which include cough, dyspnea (shortness of breath), and fever. It is not uncommon for this infection to spread to the pleura or chest wall.