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Taq polymerase exonuclease is a domain found in the amino-terminal of Taq DNA polymerase I (thermostable). It assumes a ribonuclease H-like motif . The domain confers 5' -3' exonuclease activity to the polymerase.
Topoisomerase-based cloning (TOPO cloning) is a molecular biology technique in which DNA fragments are cloned into specific vectors without the requirement for DNA ligases.Taq polymerase has a nontemplate-dependent terminal transferase activity that adds a single deoxyadenosine (A) to the 3'-end of the PCR products.
As the Taq polymerase extends the primer and synthesizes the nascent strand (from the single-stranded template), the 5' to 3' exonuclease activity of the Taq polymerase degrades the probe that has annealed to the template. Degradation of the probe releases the fluorophore from it and breaks the proximity to the quencher, thus relieving the ...
The insert is created by PCR using Taq polymerase. This polymerase lacks 3' to 5' proofreading activity and, with a high probability, adds a single, 3'-adenine overhang to each end of the PCR product. It is best if the PCR primers have guanines at the 5' end as this maximizes probability of Taq DNA polymerase adding the terminal adenosine ...
Similar effects are also achieved with mixtures of thermostable DNA polymerases of both types with a mixing ratio of the enzyme activities of type A and B polymerases of 30 to 1, [22] [36] e.g. Herculase [8] and TaqPlus [10] as a commercial mixture of Taq and Pfu polymerase, Expand as a commercial mixture of Taq and Pwo, [37] Expand High ...
[2] [12] Platinum Taq DNA polymerase and AccuStart Taq DNA polymerase ( both developed by Ayoub Rashtchian at Life technologies and Quanta BioSciences, respectively) are examples of commercially available antibody based hot start Taq DNA polymerases. These Taq DNA polymerase are precomplexed with a mixture of monoclonal antibodies specific to ...
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