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The interaction overview diagram is similar to the activity diagram, in that both visualize a sequence of activities. The difference is that, for an interaction overview, each individual activity is pictured as a frame which can contain a nested interaction diagram. This makes the interaction overview diagram useful to "deconstruct a complex ...
Column chromatography in chemistry is a chromatography method used to isolate a single chemical compound from a mixture. Chromatography is able to separate substances based on differential absorption of compounds to the adsorbent; compounds move through the column at different rates, allowing them to be separated into fractions.
A sample DSM with 7 elements and 11 dependency marks. The design structure matrix (DSM; also referred to as dependency structure matrix, dependency structure method, dependency source matrix, problem solving matrix (PSM), incidence matrix, N 2 matrix, interaction matrix, dependency map or design precedence matrix) is a simple, compact and visual representation of a system or project in the ...
Silica gel particles are commonly used as a stationary phase in high-performance liquid chromatography (HPLC) for several reasons, [13] [14] including: High surface area: Silica gel particles have a high surface area, allowing direct interactions with solutes or after bonding of variety of ligands for versatile interactions with the sample molecules, leading to better separations.
Interaction overview diagram; Timing diagram; A Communication diagram models the interactions between objects or parts in terms of sequenced messages. Communication diagrams represent a combination of information taken from Class, Sequence, and Use Case Diagrams describing both the static structure and dynamic behavior of a system.
neg - an invalid interaction. opt - single alternative with a guard condition. par - each fragment is run in parallel. ref - an interaction defined in another diagram. strict - a fragment with the ordering of reception events across multiple lifelines follow strictly their graphical arrangement.
The stationary phase is first loaded into a column to which the mobile phase is introduced. Molecules that bind to the ligand will remain associated with the stationary phase. A wash buffer is then applied to remove non-target biomolecules by disrupting their weaker interactions with the stationary phase, while the biomolecules of interest will ...
For example, in anion-exchange columns, the ion exchangers repeal protons so the pH of the buffer near the column differs is higher than the rest of the solvent. [52] As a result, an experimenter has to be careful that the protein(s) of interest is stable and properly charged in the "actual" pH.