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The rate at which fluid is filtered across vascular endothelium (transendothelial filtration) is determined by the sum of two outward forces, capillary pressure and colloid osmotic pressure beneath the endothelial glycocalyx (), and two absorptive forces, plasma protein osmotic pressure and interstitial pressure (). The Starling equation is the ...
Diffusion occurs through the walls of the vessels due to a concentration gradient, allowing the necessary exchange of ions, molecules, or blood cells. The permeability of a capillary wall is determined by the type of capillary and the surface of the endothelial cells. A continuous, tightly spaced endothelial cell lining only permits the ...
Capillary electrophoresis (CE) is a family of electrokinetic separation methods performed in submillimeter diameter capillaries and in micro- and nanofluidic channels.Very often, CE refers to capillary zone electrophoresis (CZE), but other electrophoretic techniques including capillary gel electrophoresis (CGE), capillary isoelectric focusing (CIEF), capillary isotachophoresis and micellar ...
Some capillary techniques provide also a chamber like arrangement, however, there is no filter between the cells and the test substance. [5] Quantitative results are gained by the multiwell type of this probe using 4-8-12-channel pipettes. Accuracy of the pipette and increased number of the parallel running samples is the great advantage of ...
Exchange surface. Presence of natural barriers. These are obstacles to a drug's diffusion similar to those encountered during its absorption. The most interesting are: Capillary bed permeability, which varies between tissues. Blood-brain barrier: this is located between the blood plasma in the cerebral blood vessels and the brain's ...
Elution principle of column chromatography. In analytical and organic chemistry, elution is the process of extracting one material from another by washing with a solvent: washing of loaded ion-exchange resins to remove captured ions, or eluting proteins or other biopolymers from a gel electrophoresis or chromatography column.
Flow-induced dispersion analysis (FIDA) is an immobilization-free technology used for characterization and quantification of biomolecular interaction and protein concentration under native conditions. [1] [2] [3] In the FIDA assay, the size of a ligand (indicator) with affinity to the target analyte is measured. When the indicator interacts ...
Capillary electrophoresis–mass spectrometry (CE–MS) is an analytical chemistry technique formed by the combination of the liquid separation process of capillary electrophoresis with mass spectrometry. [1] CE–MS combines advantages of both CE and MS to provide high separation efficiency and molecular mass information in a single analysis. [2]