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The original tool was created by Ken Moore as the first optical design program specifically developed for Microsoft Windows. It was introduced in 1990 and was initially called “Max” after the programmer’s dog. The name was later changed to Zemax due to a trademark conflict.
The near-field optical (NFO) microscope involved a sub-wavelength aperture at the apex of a metal coated sharply pointed transparent tip, and a feedback mechanism to maintain a constant distance of a few nanometers between the sample and the probe. Lewis et al. were also aware of the potential of an NFO microscope at this time. [14]
Optical resolution of cellular structures in the range of about 50 nm can be achieved, even in label-free cells, using localization microscopy SPDM. By using two different laser wavelengths, SPDM reveals cellular objects which are not detectable under conventional fluorescence wide-field imaging conditions, beside making for a substantial ...
The limit of optical resolution in a conventional microscope, the so-called diffraction limit, is in the order of half the wavelength of the light used to image.Thus, when imaging at visible wavelengths, the smallest resolvable features are several hundred nanometers in size (although point-like sources, such as quantum dots, can be resolved quite readily).
With no modification to the microscope, i.e. with a simple wide field light microscope, the quality of optical sectioning is governed by the same physics as the depth of field effect in photography. For a high numerical aperture lens, equivalent to a wide aperture, the depth of field is small (shallow focus) and gives good optical sectioning.
The ability of a lens to resolve detail is usually determined by the quality of the lens, but is ultimately limited by diffraction.Light coming from a point source in the object diffracts through the lens aperture such that it forms a diffraction pattern in the image, which has a central spot and surrounding bright rings, separated by dark nulls; this pattern is known as an Airy pattern, and ...
Learn how to download and install or uninstall the Desktop Gold software and if your computer meets the system requirements.
The optical microscope, also referred to as a light microscope, is a type of microscope that commonly uses visible light and a system of lenses to generate magnified images of small objects. Optical microscopes are the oldest design of microscope and were possibly invented in their present compound form in the 17th century.