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Recently a web-based database of human and mouse housekeeping genes and reference genes/transcripts, named Housekeeping and Reference Transcript Atlas (HRT Atlas), was developed to offer updated list of housekeeping genes and reliable candidate reference genes/transcripts for RT-qPCR data normalization. [1]
The database also offers some empirically validated primers and predicted modifiers (disease and small molecules) of the expression of these reference genes. The following represent genes that should probably not be used for reference purposes: GUSB , [ 5 ] RPLP0 , [ 5 ] TFRC , [ 5 ] GAPDH , HSP90 , and β-actin .
Thus both the number and type of housekeeping genes interrogated by MLST may differ from species to species. For each of these housekeeping genes, the different sequences are assigned as alleles and the alleles at the loci provide an allelic profile. A series of profiles can then be the identification marker for strain typing.
Housekeeping and Reference Transcript Atlas (HRT Atlas) [27] web-based tool for searching cell specific candidate reference genes/transcripts suitable for qPCR experiment normalization. HRT Atlas also describes a complete list of human and mouse housekeeping genes and transcripts
CpG islands typically occur at or near the transcription start site of genes, particularly housekeeping genes, in vertebrates. [14] A C (cytosine) base followed immediately by a G (guanine) base (a CpG) is rare in vertebrate DNA because the cytosines in such an arrangement tend to be methylated.
Andy Cohen is spilling the tea on what it's like working with longtime friend and colleague Anderson Cooper. Before SiriusXM's 10th Annual Radio Andy Holiday Hangout (which he co-hosts with Amy ...
[4] [5] For both bacteria and archaea the 16S rRNA/rDNA gene is used. It is a common housekeeping gene in all prokaryotic organisms and therefore is used as a standard barcode to assess prokaryotic diversity. For protists, the corresponding 18S rRNA/rDNA gene is used. [6]
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