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Toluidine blue is also commonly used to stain frozen sections (rapid microscopic analysis of a specimen). Because time is of the essence for a frozen section, toluidine blue allows for the frozen section to be stained and reviewed in 10 to 20 seconds. [4] The other staining method for frozen sections (rapid H&E) takes approximately 60 to 90 ...
The H&E staining procedure is the principal stain in histology [3] [7] [2] [5] in part because it can be done quickly, [7] is not expensive, and stains tissues in such a way that a considerable amount of microscopic anatomy [9] [10] is revealed, [7] [5] [4] and can be used to diagnose a wide range of histopathologic conditions. [8]
The absence of color change in staining is named orthochromasia. The underlying mechanism for metachromasia requires the presence of polyanions within the tissue. When these tissues are stained with a concentrated basic dye solution, such as toluidine blue, the bound dye molecules are close enough to form dimeric and polymeric aggregates.
Jaswant Singh–Bhattacharji stain, commonly referred to as JSB stain, is a rapid staining method for detection of malaria. [1] [2] It is useful for the diagnosis of malaria in thick smear samples of blood. [3] The JSB stain is commonly used throughout India, but rarely used in other countries. [4]
Many methods of performing Perls Prussian blue stain for iron have been published, [2] Drury and Wallington (1980) give a protocol that uses a mixture of 1 part 2% hydrochloric acid and 1 part 2% potassium ferrocyanide that is applied to the section for 20–30 minutes followed by a rinse in distilled water and application of a counterstain ...
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The detection limit for phosphoproteins is below 1 ng after one hour of staining, [4] for anionic polysaccharides between 10 and 500 ng. [5] [6] Highly anionic proteins are stained blue, proteoglycans purple and anionic proteins pink. [7] RNA is stained blueish-purple with a detection limit of 90 ng and DNA is stained blue with a detection ...
They appear reddish when stained with polychrome methylene blue or toluidine blue (metachromasia). Special staining techniques such as Albert's or Neisser's demonstrate the granules more clearly. Volutin granules are characteristically present in diphtheria bacilli. Their function is uncertain.