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Frozen section procedure: tissue embedded in optimal cutting temperature compound, mounted on a chuck in a cryostat and ready for section production. Optimal cutting temperature (OCT) compound is used to embed tissue samples prior to frozen sectioning on a microtome-cryostat. This process is undertaken so as to mount slices (sections) of a ...
The frozen section procedure as practiced today in medical laboratories is based on the description by Dr Louis B. Wilson in 1905. Wilson developed the technique from earlier reports at the request of Dr William Mayo, surgeon and one of the founders of the Mayo Clinic [3] Earlier reports by Dr Thomas S. Cullen at Johns Hopkins Hospital in Baltimore also involved frozen section, but only after ...
A cryostat (from cryo meaning cold and stat meaning stable) is a device used to maintain low cryogenic temperatures of samples or devices mounted within the cryostat. Low temperatures may be maintained within a cryostat by using various refrigeration methods, most commonly using cryogenic fluid bath such as liquid helium . [ 1 ]
Vaporized hydrogen peroxide (trademarked VHP, [1] also known as hydrogen peroxide vapor, HPV) is a vapor form of hydrogen peroxide (H 2 O 2) with applications as a low-temperature antimicrobial vapor used to decontaminate enclosed and sealed areas such as laboratory workstations, isolation and pass-through rooms, [2] and even aircraft interiors.
However, dry cryostats have high energy requirements and are subject to mechanical vibrations, such as those produced by pulse tube refrigerators. The first experimental machines were built in the 1990s, when (commercial) cryocoolers became available, capable of reaching a temperature lower than that of liquid helium and having sufficient ...
After use of a carcinogen or if exposure to a carcinogen has occurred, ensure the correct decontamination procedure is executed. Personnel must wash hands and arms with soap and water, immediately after handling. If exposure occurs, contact emergency health services and use safety shower/eye wash station.
Dry decontamination is a relatively recent method of decontamination and is especially useful in cold weather conditions, [2] or when water is scarce or difficult to transport. Dry decontamination reduces the size and manpower requirements of the decontamination line and eliminates the need to purchase excess equipment that becomes ineffective ...
Decontamination of humans is usually done by a three-step procedure, separated by sex: removal of clothing, washing, and reclothing. People suspected of being contaminated are usually separated by sex, and led into a decontamination tent, trailer, or pod, where they shed their potentially contaminated clothes in a strip-down room.