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Golden Gate assembly involves digesting DNA sequences containing a type IIS restriction enzyme cut site and ligating them together. Golden Gate Cloning or Golden Gate assembly [1] is a molecular cloning method that allows a researcher to simultaneously and directionally assemble multiple DNA fragments into a single piece using Type IIS restriction enzymes and T4 DNA ligase. [2]
The Golden Braid assembly standard also builds on the first tier of Golden Gate assembly and assembles further tiers via a pairwise protocol. Four tier one destination vectors (assembled via Golden Gate assembly) are assembled into two tier 2 destination vectors, which are then used as tier 3 entry vectors for the tier 3 destination vector.
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Golden Gate assembly: Software used: Serif Affinity Designer (Mar 31 2020) Conversion program: PDFlib+PDI 9.1.2p1-i (OS X/macOS) Encrypted: no: Page size: 468 x 720 pts:
The Silver Veil and the Golden Gate is a late period, coastal landscape painting by American Impressionist Childe Hassam. Completed in 1914 during one of his visits to California, the piece depicts the Golden Gate Strait, a narrow passage connecting the San Francisco Bay to the Pacific Ocean , as seen near Sausalito .
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