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DPPH has two major applications, both in laboratory research: one is a monitor of chemical reactions involving radicals, most notably it is a common antioxidant assay, [1] and another is a standard of the position and intensity of electron paramagnetic resonance signals.
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Immunoperoxidase is a type of immunostain used in molecular biology, medical research, and clinical diagnostics.In particular, immunoperoxidase reactions refer to a sub-class of immunohistochemical or immunocytochemical procedures in which the antibodies are visualized via a peroxidase-catalyzed reaction.
Antibody testing has become widely available. It can be done for individual viruses (e.g. using an ELISA assay) but automated panels that can screen for many viruses at once are becoming increasingly common. [citation needed]
For each LAMP assay, primers must be specifically designed to be compatible with the target DNA. This can be difficult which discourages researchers from using the LAMP method in their work. [ 1 ] There is however, a free software called Primer Explorer, developed by Fujitsu in Japan, which can aid in the selection of these primers.
Most commonly, antioxidant capacity is measured using the ABTS Decolorization Assay. Other antioxidant capacity assays which use Trolox as a standard include the diphenylpicrylhydrazyl ( DPPH ), oxygen radical absorbance capacity ( ORAC ) and ferric reducing ability of plasma ( FRAP ) assays.
Molecular diagnostics uses in vitro biological assays such as PCR-ELISA or Fluorescence in situ hybridization. [19] [20] The assay detects a molecule, often in low concentrations, that is a marker of disease or risk in a sample taken from a patient.
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